中成药2018，Vol.40Issue(4)：777-782,6.DOI:10.3969/j.issn.1001-1528.2018.04.003
滋肾育胎丸对复发性流产小鼠调节因子表达的影响
Effects of Zishen Yutai Pills on the expressions of regulatory factors in recurrent spontaneous abortion mice
摘要
Abstract
AIM To observe the effect of Zishen Yutai Pills (Cuscutae Semen,Ginseng Radix et Rhizoma,Dipsaci Radix,etc.) on RAS,VEGF,VEGFR-2,sVEGFR-1 and MAPK in recurrent miscarriage mice,and to explore its mechanism.METHODS CBA/J female mice + DBA/2 male mice,and CBA/J female mice + BALB/c male mice were mated by 2 females and 1 male in cage to establish the RSA model and the normal pregnancy CBA × BALB/c mouse model respectively.Since the zeroth day of pregnancy,a total of 24 CBA/J × DBA/2 mice were randomly divided into model control group,Zishen Yutai Pills group and progesterone capsule group,and 10 CBA × BALB/c mice were used as normal pregnancy control group.Mice of all groups after the respective 15-day intervention had their rate of uterine embryo loss measured and calculated.Their pathological changes of decidual tissue were determined by HE staining,their RAS,VEGF,VEGFR-2,sVEGFR-1,MAPK protein and mRNA expressions were detected by immunohistochemistry and real-time PCR.RESULTS Zishen Yutai Pills significantly reduced the rate of embryo loss and improved pathological changes of decidual tissue in RSA mice through regulating mouse decidual tissue angiogenesis and recasting,as revealed by the lowered levels of RAS,VEGF,VEGFR-2 and MAPK,and increased expression of sVEGFR-1.CONCLUSION Zishen Yutai Pills can lower the rate of embryo loss and improve decidual angiogenesis in RSA mice through altering the expression of RAS,VEGF,VEG-FR-2,sVEGFR-1 and MAPK.关键词
滋肾育胎丸/复发性流产/RAS蛋白/血管内皮生长因子Key words
Zishen Yutai Pills/recurrent spontaneous abortion/RAS protein/vascular endothelial growth factor分类
医药卫生引用本文复制引用
储继军,王瑞雪,余欣慧,刘会会,李伟莉,韩燕全..滋肾育胎丸对复发性流产小鼠调节因子表达的影响[J].中成药,2018,40(4):777-782,6.基金项目
罗元恺滋肾育胎丸中青年科研基金项目(140206) （140206）
