中成药2018，Vol.40Issue(4)：788-795,8.DOI:10.3969/j.issn.1001-1528.2018.04.005
桑白皮对肾源性水肿大鼠的代谢组学研究
Metabolic study of Mori Cortex on nephrogenic edema in rats
摘要
Abstract
AIM To conduct a metabolic research for a better understanding of nephrogenic edema and to assess the integral efficacy of Mori Cortex in rat model.METHODS The serum creatinine,urea nitrogen,albumin and urinary protein levels in rats were detected.UPLC-QTOF-MS was used to detect the urine metabolites changes,Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA)were used to screen potential biomarkers,after whose quantification,Mev software was adopted for heat map draw-ing and hierarchical cluster analysis.RESULTS The model rats manifested significantly increased levels of serum creatinine,urea nitrogen and urinary protein,decreased albumin level,and an obviously excessive amino acid metabolism as well.The 41 identified biomarkers were mainly related to disturbances in phenylalanine,pyrimidine,arginine and proline,glycine,serine and threonine,tryptophan,cysteine and methionine metabolism,and biosynthesis of pantothenic acid and coenzyme A.A reversal trend in aforementioned levels of biochemical indexes and most biomarkers due to the intervention by Mori Cortex signaled an improvement in the metabolic disorder,renal dysfunction and edema.CONCLUSION The metabolic study demonstrates the pathological status of nephrogenic edema and assesses the effect of Mori Cortex from an overall perspective,highlighting a new approach for illustrating Chinese medical syndrome and the underlying mechanism in the management of traditional Chinese medicine.关键词
桑白皮/肾源性水肿/代谢组学研究/热度图/代谢通路Key words
Mori Cortex/nephrogenic edema/metabolic study/heat map/metabolic pathway分类
医药卫生引用本文复制引用
周宁,郑晓珂,王秋红,匡海学,冯卫生,牛艳..桑白皮对肾源性水肿大鼠的代谢组学研究[J].中成药,2018,40(4):788-795,8.基金项目
国家重点基础研究发展计划(973计划)(2013CB531802) （973计划）
