中成药2018，Vol.40Issue(4)：830-834,5.DOI:10.3969/j.issn.1001-1528.2018.04.012
金钗石斛中生物碱与多糖联合酶提工艺的优化
Optimization of combined enzymatic extraction for alkaloids and polysaccharides from Dendrobium nobile
摘要
Abstract
AIM To optimize the combined enzymatic extraction for alkaloids and polysaccharides from Dendrobium nobile Lindl..METHODS With enzyme consumption,enzymolysis temperature,enzymolysis time and solidliquid ratio as influencing factors,contents of dendrobine,total alkaloids and polysaccharides as evaluation indices,orthogonal test was applied to optimizing the combined enzymatic extraction.RESULTS The optimal conditions for papain extraction were determined to be 0.10 g for enzyme consumption,45 ℃ for enzymolysis temperature,2 h for enzymolysis time,and 1 ∶ 50 for solid-liquid ratio,the contents of dendrobine,total alkaloids and polysaccharides were 3.495 5,4.341 8 and 35.898 7 mg/g,respectively.The optimal conditions for cellulase extraction were determined to be 0.30 g for enzyme consumption,50 ℃ for enzymolysis temperature,2 h for enzymolysis time,and 1 ∶40 for solid-liquid ratio,the contents of three constituents were 3.514 8,4.351 3 and 36.331 2 mg/g,respectively.The optimal conditions for pectinase extraction were determined to be 0.45 g for enzyme consumption,55 ℃ for enzymolysis temperature,2.5 h for enzymolysis time,and 1 ∶ 40 for solid-liquid ratio,the contents of three constituents were 3.524 4,4.452 8 and 26.324 2 mg/g,respectively.CONCLUSION This stable and reliable method can be used for the rapid combined enzymatic extraction for alkaloids and polysaccharides from D.nobile.关键词
金钗石斛/生物碱/多糖/联合酶提/正交试验Key words
Dendrobium nobile Lindl./alkaloids/polysaccharides/combined enzymatic extraction/orthogonal test分类
医药卫生引用本文复制引用
敖娇,鲍家科,夏玉吉..金钗石斛中生物碱与多糖联合酶提工艺的优化[J].中成药,2018,40(4):830-834,5.基金项目
贵州省中药标准化技术研究中心项目(黔科合社字[2008] 5002) （黔科合社字[2008] 5002）
