摘要
Abstract
AIM To establish an HPLC-DAD method for the simultaneous content determination of six constituents in Maiwei Dihuang Pills (Ophiopogonis Radix,Schisandrae chinensis Fructus,Rehmanniae Radix Praeparata,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent ZORBAX SB-C18column (4.6 mm × 150 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.2％ phosphate acid) flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelengths were set at 220,230,236 and 274 nm.RESULTS Deoxyschizandrin,schizandrin B,schisandrin,paeoniflorin,paeonol and loganin showed good linear relationships within the ranges of 10-70,6.5-45.5,33.5-234.5,17-119,31-217 and 34-238 μg/mL (r ＞0.990 0),whose average recoveries (RSDs) were 99.6％ (1.7％),100.4％ (1.8％),100.7％ (1.8％),102.9％ (1.7％),102.2％ (1.5％) and 99.7％ (1.2％),respectively.CONCLUSION This simple and reproducible method can be used for the rapid quality control of Maiwei Dihuang Pills.关键词
麦味地黄丸/五味子甲素/五味子乙素/五味子醇甲/芍药苷/丹皮酚/马钱苷/HPLC-DADKey words
Maiwei Dihuang Pills/deoxyschizandrin/schizandrin B/schisandrin/paeoniflorin/paeonol/loganin/HPLC-DAD分类
医药卫生
