广东海洋大学学报2018，Vol.38Issue(1)：14-21,8.DOI:10.3969/j.issn.1673-9159.2018.01.003
大豆酶解蛋白对凡纳滨对虾幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响
Effects of Enzymolytic Soybean Meal on Growth Performance, Serum Biochemical Indices, Non-specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei
摘要
Abstract
[Objectives] This study is to investigate the effects of enzymolytic soybean meal on the growth performance,serum biochemical indices,non-specific immunity and disease resistance of juvenile Litopenaeus vannamei.[Method] Eight isonitrogenous and isolipid diets were prepared by adding enzymolytic soybean meal to the final of 0,1.0％,1.5％,2.0％,2.5％,3.0％,3.5％ and 4.0％ groups of the basal diet,and fed to juvenile Litopenaeus vannamei for 56 d.[Result] The maximum weight gain rate (WGR) and specific growth rate (SGR) occurred in the 1.0％ enzymolytic soybean meal groups,and was significantly higher (P ＜ 0.05) than those of the 2.0％ to 4.0％ groups.The body crude protein content in the 1.0％ and 1.5％ groups were significantly higher than the control group (P ＜ 0.05).Protein deposition rate (PDR) in control,1.0％ and 1.5％ group were significantly higher than that in other groups (P ＜ 0.05).Muscle phosphorus content was significantly increased by dietary supplying 1.5％ to 4.0％ enzymolytic soybean meal (P＜ 0.05),and a highest value was found in 3.0％ group.Serum cholesterol (CHOL) in 1.0％,1.5％ and 2.0％ groups were significantly lower than that in the control (P ＜ 0.05).The activity of serum lysozyme (LSZ) was significantly increased by dietary supplyment of 1.5％ to 4.0％ enzymolytic soybean meal (P ＜ 0.05).The activity of serum phenoloxidase (PO) was significantly increased by dietary supplyment of 1.0％ to 3.0％ enzymolytic soybean meal (P ＜ 0.05).The activity of serum superoxide dismutase (SOD) in 1.5％ and 2.0％ group and the activity of serum acid phosphatase (ACP) in 1.0％ and 1.5％ groups were significantly higher than those in the control (P ＜ 0.05).Serum alkaline (AKP) activity was significantly increased in diets supplement with enzymolytic soybean meal (P ＜ 0.05),and the highest value occurred in the 2.5％ group.Shrimp were challenged by Vibrio harveyi for the next 7 days.The minimum cumulative mortality rate was occurred in 1.0％ group.Diets with ＞ 3.5％ enzymolytic soybean meal can inhibit the growth performance of juvenile and supplement of 1.0％ enzymolytic soybean meal can obviously improve the disease resistance of L.vannamei.[Conclusion] The growth performance of Litopenaeus vannamei can not be improved by adding enzymolytic soybean meal in the diets,and would be reduced with excessive adding (more than 3.5％ supplementation),and the supplementation of 1.0％ can obviously improve the disease resistance ofL.vannamei.关键词
大豆酶解蛋白/凡纳滨对虾/幼虾/生长性能/血清生化指标/非特异性免疫力/抗病力Key words
enzymolytic soybean meal/Litopenaeus vannamei/juveniles/growth performance/serum biochemical indices/non-specific immunity/disease resistance分类
农业科技引用本文复制引用
王铵静,杨奇慧,谭北平,肖伟伟,贾静,董晓慧,迟淑艳,刘泓宇,章双..大豆酶解蛋白对凡纳滨对虾幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响[J].广东海洋大学学报,2018,38(1):14-21,8.基金项目
农业部公益性行业科研专项(201003020) （201003020）
广东省产业技术与开发专项资金项目(2013B021100017) （2013B021100017）
广东省自然科学基金(2016A030313749) （2016A030313749）
成都美溢德生物技术有限公司项目(B16017) （B16017）
