作物学报2018，Vol.44Issue(3)：315-323,9.DOI:10.3724/SP.J.1006.2018.000315
应用SNP精准鉴定大豆种质及构建可扫描身份证
Accurate Identification of Varieties by Nucleotide Polymorphisms and Establishment of Scannable Variety IDs for Soybean Germplasm
摘要
Abstract
In order to strengthen the management of soybean germplasm and variety protection,SNP markers were developed to establish the identity of soybean varieties.A set of 23 SNP markers distributed in 13 genes were used to discriminate genotypes of 599 soybean varieties,grown in most of the soybean producing areas in China.Fourteen SNPs with high polymorphism selected from the 23 SNPs (GlySNP14) showed the improved variety identification capability,compared with any combination of 14 random SNPs.A simulated experiment confirmed that GlySNP14 could effectively distinguish of 750 soybean varieties,while the combination of random by selected from the 14 SNPs could distinguish only 361 varieties.The established ID of soybean varieties in this study contained a 38-digit serial code,which can be used for the accurate identification of soybean varieties and meet the requirements of genetic resources protection.关键词
大豆/SNP/遗传多样性/DNA指纹图谱/品种IDKey words
soybean/SNP/genetic diversity/DNA fingerprint/variety ID引用本文复制引用
魏中艳,李慧慧,李骏,Yasir A.Gamar,马岩松,邱丽娟..应用SNP精准鉴定大豆种质及构建可扫描身份证[J].作物学报,2018,44(3):315-323,9.基金项目
本研究由农产品质量安全监管(种子管理)项目(2130109)和国家农作物资源共享平台(NICGR2016大豆)项目资助.This study was supported by the project of Quality and Safety Supervision of agriculture food (Seed Management) (2130109) and National Infrastructure for Crop Germplasm Resources (NICGR2016 Soybean). （种子管理）
