作物学报2018，Vol.44Issue(3)：324-331,8.DOI:10.3724/SP.J.1006.2018.00324
水稻抗咪唑啉酮类除草剂基因ALS功能标记的开发与应用
Development and Application of the Functional Marker for Imidazolinone Herbicides Resistant ALS Gene in Rice
摘要
Abstract
Breeding and utilization of herbicide resistant rice are significant to rice production.By screening the rice germplasm,we found the herbicide resistant material "Jinjing 818".An SNP mutation G to A was present in Acetolactate synthase (ALS) gene at 1880 bp position,leading to the alteration from serine (S,AGT) to asparagine (N,AAT),which confers herbicide resistance.In this study,11 allelic-specific PCR (AS-PCR) primers were designed based on the functional mutation.After optimized these primers,we obtained two primer combinations FIN (S1/S9) and F1M (S1/S10),named AS-ALS marker.Using this marker detected the genetic population,its parents,F1 hybrid,F2 and also rice collections,inbred lines,showing that the herbicide susceptibleness allelic ALS-G could be amplified by FIN,the herbicide resistance allelic ALS-A by F1M,and heterozygous genotype by F1N and F1M simultaneously.The genotype of those tested materials perfectly matched with the phenotype of herbicide resistance or susceptibleness.Aided by AS-ALS marker selection,the homozygous ALS-A pedigrees in multi-generation backcross or self-cross showed stable herbicide resistance.Therefore,the allelic-specific PCR functional marker AS-ALS can be used in herbicide breeding efficiently,also screening herbicide resistant rice germplasm.In conclusion,the AS-ALS marker developed in this research is inexpensive and effective in breeding practice.关键词
水稻/除草剂/乙酰乳酸合成酶/ALS基因/功能标记Key words
rice (Oryza sativa L.)/herbicide/acetolactate synthase/ALS gene/functional marker引用本文复制引用
王芳权,杨杰,范方军,李文奇,王军,许扬,朱金燕,费云燕,仲维功..水稻抗咪唑啉酮类除草剂基因ALS功能标记的开发与应用[J].作物学报,2018,44(3):324-331,8.基金项目
本研究由国家重点研发计划项目(2017YFD0100400-3),江苏省现代农业重点研发项目(BE2015355),江苏省农业科学院探索性项目(ZX(17)2014),江苏省自然科学基金面上项目(BK20171326)和国家公益性行业科研专项项目(201303102)资助.This study was supported by the National Key Research and Development Program (2017YFD0100400-3),the Jiangsu Province Key Research and Development Program (Modern Agriculture,BE2015355),the Exploratory Project of the Jiangsu Academy of Agricultural Sciences (ZX(17)2014),the Natural Science Foundation of Jiangsu Province of China (BK20171326),and the Special Fund for Scientific Research on Public Causes (201303102). （2017YFD0100400-3）
