作物学报2018，Vol.44Issue(3)：332-342,11.DOI:10.3724/SP.J.1006.2018.00332
水稻类病斑突变体spl34的鉴定与基因精细定位
Identification and Gene Mapping of a Lesion Mimic Mutant spl34 in Rice (Oryza sativa L.)
摘要
Abstract
A mutant spotted leaf 34 (spl34) was screened from the progeny of indica restorer line Jinhui 10 treated with ethyl methane sulfonate (EMS).Brown lesions in spl34 exhibit on the sheath of lower leaves at the late tillering stage,then spred from the midrib to entire leaf and finally throughout the whole plant at maturity stage.Compared with the wild type,the plant height,ear length,grain number per panicle,seed setting rate and thousand-grain weight as well as the activities of protective enzymes (CAT,POD,and T-SOD) were all significantly decreased while the content of reactive oxygen species (ROS) increased in spl34.The shading assay showed that the formation of lesions in spl34 was induced by light.Histochemical analysis showed that spl34 had excessive hydrogen peroxide (H2O2) deposition and programmed cell death in the position of lesions.In addition,the chlorophyll fluorescence was weaker in spl34 than in the wild type under the fluorescence microscopy.There was no significant difference in blast resistance between spl34 and the wild type.Genetic analysis suggested that the phenotype ofspl34 was controlled by a single recessive nuclear gene,which was mapped between InDel markers LR49 and LR52 on chromosome 4 with an interval of 200 kb.Sequencing analysis revealed that a single base substitution (G to T) occurred at 3449 bp in the DNA sequence of LOC_Os04g56480,resulting in an amino acid change from tryptophane to cysteine.The qRT-PCR results showed that the transcriptional level of LOC_Os04g56480 was down-regulated in spl34,while that of some pathogenesis-related genes was highly up-regulated when compared with the wild type.关键词
水稻/类病斑突变体/spl34/基因/精细定位Key words
rice/lesion mimic mutant/spl34/gene/fine mapping引用本文复制引用
刘宝玉,刘军化,杜丹,闫萌,郑丽媛,吴雪,桑贤春,张长伟..水稻类病斑突变体spl34的鉴定与基因精细定位[J].作物学报,2018,44(3):332-342,11.基金项目
本研究由国家转基因生物新品种培育重大专项(2016ZX08001002-002)资助.This study was supported by the National Major Project for Developing New GM Crops (2016ZX08001002-002). （2016ZX08001002-002）
