作物学报2018，Vol.44Issue(3)：369-375,7.DOI:10.3724/SP.J.1006.2018.00369
一个新的玉米Vp15基因等位突变体的遗传分析与分子鉴定
Genetic Analysis and Molecular Characterization of a New Allelic Mutant of Vp15 Gene in Maize
摘要
Abstract
We identified a new maize viviparous mutant during seed reproduction,designated as vp-like4.This mutant phenotype was steadily inherited and genetically regulated by a single recessive gene.Using an F2 segregation population derived from vp-like4 and inbred line Mo17,we mapped the target gene in an interval from 173.8 to 175.6 Mb on chromosome 5 by the BSR-Seq strategy.Using genomic sequence database,we found that viviparous gene Vp15 is located in this mapping region.The maize Vp15 gene encodes the molybdopterin synthase small subunit,which is required in the process of catalyzing the reaction from carotenoid to ABA.The heterozygous plants from two independent vp15 mutants,vp15-umu1 and vp15-DR1126,were used to cross with vp-like4 heterozygous plants,showing a 3∶1 segregation ratio for normal and viviparous kernels.The genomic sequence analysis revealed that vp-like4 mutant had a 60-bp deletion in the second exon and 3'-untranslated region of Vp15 gene,which is different from vp15-umu1 and vp15-DR1126 both mutated from a Mutator transponson inserting in the second exon of Vp15 gene.Further RT-PCR analysis revealed that the expression level of vp15 was significantly lower in vp-like4.Taken together,these evidences suggest that vp-like4 is a new allele mutant from vp15.关键词
玉米/穗发芽/突变体/Vp15/基因定位Key words
maize/viviparous/mutant/Vp15/gene mapping引用本文复制引用
王瑞,张秀艳,陈阳松,杜依聪,汤继华,王国英,郑军..一个新的玉米Vp15基因等位突变体的遗传分析与分子鉴定[J].作物学报,2018,44(3):369-375,7.基金项目
本研究由国家重点研发计划项目(2016YFD0101002)和中国农业科学院创新工程专项经费资助.This study was supported by the National Key Research and Development Program of China (2016YFD0101002) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences （2016YFD0101002）
