作物学报2018,Vol.44Issue(3):385-396,12.DOI:10.3724/SP.J.1006.2018.00385
利用抗旱选择导入系定位向日葵产量性状QTL
QTL Mapping of Yield Traits Using Drought Tolerance Selected Backcrossing Introgression Lines in Sunflower
摘要
Abstract
Drought is one of the most important factors to decrease the yield of sunflower.The BC3F2 selected backcrossing introgression lines of oil sunflower population including 45 lines were developed by drought-tolerance screening for yield using Helianthus annuus K55 with excellent comprehensive characters and drought sensitivity as recurrent parent and K58 with drought-tolerance as donor parent.The genotypes of selected backcrossing introgression lines were obtained with the whole genome SSR and SNP markers.QTLs affecting five yield traits were detected under both drought stress and well watered conditions in Hohhot and Wuchuan respectively by one-way ANOVA and Chi-square test based on the Genetic Hitchhiking Effect.The QTLs detected by one-way ANOVA were grouped into three types based on their behaviors:type Ⅰ was the QTLs detected in both watered conditions,including four QTLs affecting hundred-seed weight (HSW) in Wuchuan and two QTLs affecting seed yield (SY) and three QTLs affecting filled seeds per plant (FSP) in Hohhot,which were considered to be able to directly contribute to drought tolerance;type Ⅱ was the QTLs detected only under drought stress,including 30 QTLs in Hohhot and 27 QTLs in Wuchuan;type Ⅲ was the QTLs detected only in well watered condition,including 38 QTLs in Hohhot and 64 QTLs in Wuchuan.There 274 loci were detected by chi-square test,among them 14 loci could be detected by ANOVA and chi-square test simultaneously,which might be the key loci for drought tolerance of sunflower.The results lay a foundation of efficient drought tolerance molecular breeding and provide useful drought tolerance materials for sunflower.关键词
向日葵/抗旱性/选择回交导入系/产量性状/QTL定位Key words
sunflower/drought tolerance/selected backcrossing introgression lines/yield traits/QTL引用本文复制引用
吕品,于海峰,侯建华..利用抗旱选择导入系定位向日葵产量性状QTL[J].作物学报,2018,44(3):385-396,12.基金项目
本研究由国家自然科学基金项目(31160288)资助.This study was supported by the National Natural Science Foundation of China (31160288). (31160288)