中国现代中药2018，Vol.20Issue(4)：377-381,5.DOI:10.13313/j.issn.1673-4890.20171208005
DNA条形码技术在新疆进口民族民间药材基原考证中的应用
Application of DNA Barcoding Technology in Studying the Original Species of Imported Ethnical Medicinal Materials in Xinjiang
摘要
Abstract
Objective:To explore the validity of using DNA barcoding technology to identify the original species of im-ported ethnical medicinal materials in Xinjiang.Methods:Total DNA was extracted by DNA extraction kit from thirty samples, ITS2 and psbA-trnH sequences were amplified by PCR methord and bidirectional sequenced.Sequence informations were analysed by MEGA7.0 software,all ITS2 and psbA-trnH sequences were identified by comparison of sequence similarity in professional nu-cleic acid sequence database,the most similar plant sequence were searched to determine the original species.Results:Accord-ing to ITS2 and psbA-trnH sequences,twenty-eight medicinal samples obtained effective sequences.The comparison of sequence similarity results showed that fifteen kinds of medicinal materials were consistent with the original species as recorded in the litera-ture,seven samples were closely related to their original species,and six samples were different with the original species as re-corded.Conclusion:It is urgent to investigate and study the original species of imported Uygur medicinal materials.关键词
进口药材/DNA条形码/ITS2/psbA-trnH/基原植物Key words
Imported medicinal materials/DNA barcode/ITS2/psbA-trnH/original species引用本文复制引用
李亚伟,刘强,樊丛照,李晓瑾,刘富铭..DNA条形码技术在新疆进口民族民间药材基原考证中的应用[J].中国现代中药,2018,20(4):377-381,5.基金项目
国家质量监督检验检疫总局科技计划项目(2016IK262) （2016IK262）
乌鲁木齐市科技局项目(G151010003) （G151010003）
