中国现代中药2018，Vol.20Issue(4)：395-401,7.DOI:10.13313/j.issn.1673-4890.20171104002
龟甲中具有潜在药理活性的miRNA初步筛选
Study on Preliminary Screening of miRNAs with Potential Pharmacological Activity in Plastrum Testudinis
摘要
Abstract
Objective:To extract and sequence the miRNA in Plastrum Testudinis,and screen preliminarily the po-tential pharmacological activity.Methods:Transcriptome analyses were carried out on Plastrum Testudinis,and the miRNAs which have counts of more than 10 000 were selected.The copy number of these miRNAs in Plastrum Testudinis,Chinemys reevesii,Mauremys mutica were measured respectively by qRT-PCR.Results:3 miRNAs were selected from the transcriptome data:xtr-miR-22,NW-006642957-1-500 and NW-006622633-1-195.The results of qRT-PCR showed that the copy numbers of xtr-miR-22 in Plastrum Testudinis,Chinemys reevesii were significantly higher than that of the counterpart in Mauremys muti-ca. The target gene prediction confirmed that xtr-miR-22 target expression of PML Gene.Conclusion:The miRNAs do exist in Plastrum Testudinis after deep-processing,and xtr-miR-22 which was discovered in Plastrum Testudinis have potential pharma-cological activity to treat promyelocytic leukemia.关键词
龟甲/miRNA/筛选研究Key words
Plastrum Testudinis/miRNA/screening studies引用本文复制引用
陈铭阳,胡小松,许贞,任广喜,芦海生,刘春生..龟甲中具有潜在药理活性的miRNA初步筛选[J].中国现代中药,2018,20(4):395-401,7.基金项目
公益性行业科研专项(201507002-4-2) （201507002-4-2）
