中国现代中药2018，Vol.20Issue(4)：402-408,7.DOI:10.13313/j.issn.1673-4890.20170831006
大黄素对CCl4诱导小鼠肝纤维化的作用机制
Mechanism Study of Effect of Emodin Against Liver Fibrosis Induced by CCl4in Mice
摘要
Abstract
Objective:To observe the therapeutic effect of emodin for CCl4-induced liver fibrosis and explore the pos-sible mechanism.Methods:24 male C57B6 mice were randomly divided into blank group,CCl4group and emodin group, and there were eight mice in each group.The Hepatic fibrosis was induced by intraperitoneal CCl4injection.The mice of emodin group received emodin via oral administration at a dosage of 40 mg·kg-·1d-1at the same time.Liver inflammation and fibrosis were observed by HE staining,Masson staining and α-Sma immunohistochemistry.CD45 +,CD11B+and F4/80 +cells in liver tissues were observed by immunohistochemistry.TGF-β1 mRNA expression were detected by real time PCR.Results:Mice of CCl4group and emodin group were injected with CCl4for 4 weeks.The pathological changes of liver cells and fibrosis were ob-served,which suggested that modeling succeeded.Compared with the CCl4group,the liver inflammation and hepatic fibrosis were significantly decreased.Intraperitoneal injection of CCl4increased the number of CD45 +,CD11B+and F4/80 +cells ex-pression in the liver tissue of mice.And emodin significantly reduced the number of these three types of cells,the correlation value was statistically significant.In addition,Compared to the blank group,the expression of TGF-β1 was significantly in-creased in CCl4group,while emodin reduced the CCl4-induced expression of TGF-β1,the correlation value was statistically significant.Conclusion:Emodin can reduce CCl4induced liver fibrosis,reduce liver fibrosis factor-TGF-β1 release,and its mechanism may be through the reduction of infiltration of monocytes-derived macrophages.关键词
肝纤维化/大黄素/TGF-β1/单核细胞/巨噬细胞Key words
Liver fibrosis/emodin/TGF-β1/monocyte/macrophages引用本文复制引用
王云龙,郭海,魏睦新..大黄素对CCl4诱导小鼠肝纤维化的作用机制[J].中国现代中药,2018,20(4):402-408,7.基金项目
江苏省中医药局科技项目(YB2015163) （YB2015163）
