中国现代中药2018，Vol.20Issue(4)：409-414,6.DOI:10.13313/j.issn.1673-4890.20170907004
华蟾素对乳腺癌MDA-MB-231细胞增殖迁移凋亡的影响
Effect of Cinobufacini on MDA-MB-231 Cells Proliferation,Migration and Apoptosis
摘要
Abstract
Objective:To investigate the effect of cinobufacini on MDA-MB-231 cells proliferation,migration and apoptosis,and detect the underlying mechanism.Methods:MDA-MB-231 cells were treated with different concentrations of cinobufacini,the morphological changes of MDA-MB-231 cells were observed under inverted microscope.CCK-8 assay was used to detect the effect of cinobufacini on MDA-MB-231 cell proliferation.Scratches test was employed to investigate cell mi-gration in MDA-MB-231 cells and cell apoptosis was analyzed by flow cytometry with the staining of Annexin V-FITC/PI.Western Blot was employed to detect the expression level of Bax,Bcl-2,Cleaved capsase-3,Cleaved caspase-9 and PTHrP.Results:CCK-8 results showed that cinibufacini could inhibit the proliferation of MDA-MB-231 cells in a concentration and time-dependent manner.Scratches test confirmed that cinibufacini treatment reduced the migration ability of MDA-MB-231 cells.The results of flow cytometry suggested that cinibufacini could induce apoptosis in MDA-MB-231 cells in a concentration dependent manner.The expression levels of Bax,Cleaved capsase-3,Cleaved caspase-9 were increased,whereas the expres-sion levels of Bcl-2 and PTHrP were decreased with the treatment of cinobufacini.Conclusion:Cinobufacini could induce ap-optosis through the mitochondrial apoptotic pathway to exert anti-tumor effect on MDA-MB-231 cells.The effect of cinobufacini on breast cancer osteolytic bone metastasis needs to be further studied in vivo.关键词
华蟾素/MDA-MB-231细胞/凋亡/PTHrPKey words
Cinobufacini/MDA-MB-231 cell/apoptosis/PTHrP引用本文复制引用
郑迪,代国,张政佩,陶春杰,余铃,郭卫春..华蟾素对乳腺癌MDA-MB-231细胞增殖迁移凋亡的影响[J].中国现代中药,2018,20(4):409-414,6.基金项目
国家自然科学基金项目(81341078) （81341078）
