中国现代中药2018，Vol.20Issue(4)：415-420,431,7.DOI:10.13313/j.issn.1673-4890.20170804001
白芍总苷对脑缺血大鼠学习记忆能力及海马CA1区神经元的保护作用
Effect of Total Glucosides of Paeony on Learning and Memory Ability and Protective Effect of Neurons in Hippocampal CA1 Region of Cerebral Ischaemia Rats
摘要
Abstract
Objective:To study the effect of total glucosides of paeony on learning and memory ability of cerebral is-chemia rats and the protective effect on neurons in hippocampal CA1 region and explore its mechanism.Methods:The cerebral ischemia model was established by ligating bilateral common carotid artery,the total glucosides of paeony(50,100, 200 mg·kg-1)were administered by intragastric administration for 28 days on the second day after operation,once a day.After 28 d,Morris water maze test and platform test were used to evaluate the learning and memory ability of rats.The morphological changes of neurons in hippocampus CA1 region were observed by HE staining,the cell apoptosis were observed after TUNEL staining,the expression of Bcl-2 and Bax were examined by IHC,the activity of antioxidant enzymes and malon-dialdehyde(MDA)in hippocampus tissue were measured.Results:The total glucosides of paeony(100,200 mg·kg-1) could significantly shorten the time of escape latency in cerebral ischemia rats,significantly shorten the time of platform latency and increase the number of jumpers(P<0.05 or P<0.01),significantly improve hippocampal CA1 neuronal pathological changes and apoptosis,significantly decrease the apoptosis index(AI)(P<0.01),significantly up-regulate the expression of Bcl-2 and down-regulated the expression of Bax,increase the Bcl-2/Bax ratio(P<0.05 or P<0.01),significantly im-prove antioxidant enzymes(SOD,CAT,GSH-Px)activity and decrease MDA content(P<0.05 or P<0.01).Conclusion:The total glucosides of paeony have improvement effects on the ability of learning and memory in cerebral ischemia rats,which perhaps related to inhibit the neuronal lesions in hippocampal CA1 neurons,alter the expression of Bcl-2 and Bax,suppress hippocampal CA1 neurons apoptosis.关键词
白芍总苷/脑缺血/学习/记忆/海马Key words
Total glucosides of paeony/cerebral ischemic/learning/memory/hippocampal引用本文复制引用
史晴晴,黄宁,郭洋洋..白芍总苷对脑缺血大鼠学习记忆能力及海马CA1区神经元的保护作用[J].中国现代中药,2018,20(4):415-420,431,7.基金项目
廊坊市科技支撑计划项目(2016013151) （2016013151）
