中国现代中药2018，Vol.20Issue(4)：421-425,5.DOI:10.13313/j.issn.1673-4890.20170724001
基于ATR-FTIR成像技术的川贝母粉末显微光谱鉴定
Microspectroscopic Identification of Fritillariae Cirrhosae Bulbus Powder by ATR-FTIR Microspectroscopic Imaging
摘要
Abstract
Objective:To establish a simple and rapid identification method for identification different kinds of Fritil-lariae Bulbus powder and ensure the authenticity of Fritillariae Cirrhosae Bulbus(FCB).Methods:The powder of different kinds of Fritillariae Bulbus was measured directly by ATR-FTIR microspectroscopic imaging.Principal component analysis and the normalized relative peak intensity method were used for the qualitative and quantitative interpretation of the microscopic ATR-FTIR spectra to find out the discriminative spectral features between FCB and other kinds of Fritillariae Bulbus.Results:Three kinds of microspectroscopic pixels,corresponding to starch grains,protein and cell wall,can be found in the ATR-FT-IR image of each sample.Meanwhile,the number of the pixels corresponding to starch grains was the largest in FCB.The nor-malized relative peak intensity Rmwas calculated using the band area in the region of 1200-900 cm-1as the target peak and the band area in the region of 1700-1500 cm-1as the reference peak.Rmfor FCB was always about 0.70,which was signifi-cantly larger than Rmfor other kinds of Fritillariae Bulbus.The Rmvalue can be used as the quantitative criterion to discriminate the FCB powder from the powder of other kinds of Fritillariae Bulbus.Conclusion:The microspectroscopic identification meth-od based on the ATR-FTIR imaging technique can be used for the simple,rapid,objective and quantitative identification of FCB.关键词
贝母/川贝母/显微鉴定/显微红外光谱/衰减全反射成像Key words
Fritillaria/Fritillariae Cirrhosae Bulbus/microscopic identification/FTIR microspectroscopy/ATR imaging引用本文复制引用
王月,刘傲雪,张久旭,李小阳,陈建波,王晶娟..基于ATR-FTIR成像技术的川贝母粉末显微光谱鉴定[J].中国现代中药,2018,20(4):421-425,5.基金项目
国家自然科学基金(81303217) （81303217）
