中国现代中药2018，Vol.20Issue(4)：453-457,5.DOI:10.13313/j.issn.1673-4890.20170510005
百药煎中转化鞣质生成没食子酸的最佳菌种组合筛选
Screening Optimal Strains Combination for Transforming Tannin into Gallic acid in Chinese gall leaven
摘要
Abstract
Objective:To screen the optimal strain combination for the fermentation production of gallic acid from tannin in Chinese gall leaven.Methods:A variety of microorganisms with the ability to degrade tannin from traditional pro-cessed Chinese gall leaven were used as starting strains,and using single bacteria and mixed bacteria fermentation methods and HPLC determination,the ability of transforming tannin to gallic acid was assessed.Results:The optimal strains combination for the synergistic transformation of Chinese gall leaven producing gallic acid was the four strains(HMB5,HMB2,HMY1 and HMY2)combination,Using this combination fermentation,the amount of gallic acid in samples reached 0.59 g·g-1sample, 13% more than that of the traditional processed Chinese gall leaven(0.52 g·g-1).More over,the antibacterial activity of Chi-nese gall leaven by mixed strains fermentation were better than those of the traditional processed.Conclusion:The optimal strains combination obtained here is better than traditional processed Chinese gall leaven and can serve the construction of mod-ern Chinese gall leaven processing technology.关键词
百药煎/没食子酸/固态发酵/最优混菌组合Key words
Processed Chinese gall leaven/gallic acid/solid-state fermentation/optimal strains combination引用本文复制引用
胡梦,程玉冰,张培燕,刘英,胡海峰..百药煎中转化鞣质生成没食子酸的最佳菌种组合筛选[J].中国现代中药,2018,20(4):453-457,5.基金项目
国家中医药管理局中医药行业专项(201507004) （201507004）
