安徽农业科学2018，Vol.46Issue(12)：4-6,26,4.
响应面法优化三百棒多糖提取工艺
Optimization of Extracting Polysaccharide from Toddalia asiatica Lam.by the Response Surface Method
摘要
Abstract
[Objective]Response surface methodology was used to optimize the extraction condition of polysaccharide from Toddalia asiatica Lam..[Method]The effects of solid-liquid ratio,extraction temperature,extraction time and extraction times by single factor experiments were studied.Subsequently,based on the four factors,a polysaccharide yield regression model was established using the Box-Behnken design.A regres-sion model equation was fitted.[Result]The optimum extractions were solid-liquid ratio of 1:15(g:mL),extraction temperature of 93 ℃,extrac-tion time of 4 h,extraction times of 3.Under the conditions, the maximum experimental polysaccharide yield was 4.75%,which was basically consisitent with the predictied of 4.99%.[Conclusion]The optimized technology was feasible,and had high application value.关键词
三百棒/多糖/响应面/提取工艺Key words
Toddalia asiatica Lam./Polysaccharide/Response surface/Extraction分类
农业科技引用本文复制引用
田瑞,夏燕,冯佳,杨年安,向阳,陈安平,袁林..响应面法优化三百棒多糖提取工艺[J].安徽农业科学,2018,46(12):4-6,26,4.基金项目
国家自然科学基金项目(81360672) （81360672）
湖北省民委项目(HBM W2012027). （HBM W2012027）
