安徽农业科学2018，Vol.46Issue(12)：7-10,4.
图像辅助流式细胞仪在鲫鱼血细胞分析中的应用
Application of Image-based Flow Cytometry on Blood Cell Analysis of Carassius auratus
摘要
Abstract
[Objective]The aim was to explore application of image-based flow cytometry on blood cell analysis of Carassius auratus.[Method] Image-based flow cytometry was introduced into the automatic classification analysis of Carassius auratus blood cells,and then applied to the affect analysis of copper ion on C.auratus blood cells.[Result]The image-based analysis function greatly improved gating efficiency for distinguish the leucocyte population from huge amount of erythrocytes,which possess unremovable nuclei.1.18%-1.43%of whole blood cells were leucocytes. 120 mg/L Cu2 +stress could cause serious decline of blood cell counting.This decline resulted from cell swelling and cell injury caused by stimu-lation of high concentration Cu2 +.[Conclusion]In this research,an automatic method for efficiently distinguish C.auratus leucocyte is estab-lished.It will facilitate the blood cell analysis of fish.关键词
图像辅助流式细胞仪/鲫鱼/血细胞/铜离子/毒性Key words
Image-based flow cytometry/Carassius auratus/Blood cell/Copper ion/Toxicity分类
农业科技引用本文复制引用
张树花,刘逸尘,胡锦丽,任星潮,晋伟,张亦陈..图像辅助流式细胞仪在鲫鱼血细胞分析中的应用[J].安徽农业科学,2018,46(12):7-10,4.基金项目
国家自然科学基金项目(31472299) （31472299）
天津市应用基础与前沿技术研究计划项目(15JCZDJC33800) （15JCZDJC33800）
天津市水产生态及养殖重点实验室开放基金项目(TJAE2015005) （TJAE2015005）
"十二五"农村领域国家科技计划课题(2011BAD13B04). （2011BAD13B04）
