北京师范大学学报（自然科学版）2017，Vol.53Issue(6)：668-673,6.DOI:10.16360/j.cnki.jbnuns.2017.06.007
通过S-柠檬烯合酶突变体的动力学分析揭示萜烯合酶进化的机制
Kinetics of S-limonene synthase mutants reveals a novel evolutionary mechanism of terpene synthases
摘要
Abstract
Terpene synthases have high specificity through divergent evolution,but how specificity evolved is not clear.In the present work,kinetics features of wild type and mutant S-limonene synthases were analyzed by colorimetric assay with malachite green as substrate.Compared with wild type enzyme,mutant increase in Km and decrease in kcat were found to be small.Kinetics data indicated that mutant terpene synthases which produce new terpene products still retain much of their overall activities.This character provides a novel mechanism for new monoterpene synthase evolution from existing enzymes and for diversification of terpenoids.The present work provides a model for terpene synthase evolution in nature,and offers solution to remodel terpene synthase to improve productivity of terpene biosynthesis.关键词
单萜烯合酶/柠檬烯合酶/孔雀绿/动力学/分子进化Key words
monoterpene synthase/limonene synthase/malachite green/kinetics/molecular evolution分类
农业科技引用本文复制引用
许金坤,艾莹,徐经纬,AHMED Roba,王建辉,佟丽,杨冬..通过S-柠檬烯合酶突变体的动力学分析揭示萜烯合酶进化的机制[J].北京师范大学学报（自然科学版）,2017,53(6):668-673,6.基金项目
国家自然科学基金资助项目(31070682,31640004) （31070682,31640004）
北京市自然科学基金资助项目(5154029) （5154029）
霍英东教育基金会资助项目(132025) （132025）
