草业科学2018,Vol.35Issue(4):839-847,9.DOI:10.11829/j.issn.1001-0629.2017-0268
内生真菌发酵液浸种对干旱胁迫下黑麦草种子萌发的影响
Effect of seed soaking with E pichlo?fermentation broth on germination characteristics in perennial ryegrass under drought stress
摘要
Abstract
To explore the in vitro effect of Epichlo?on the drought resistance in perennial ryegrass seeds during germination,the seeds were soaked in three species of Epichlo?(E.gansuensis,E.bromicola,and Epichlo? sp.)fermentation broth,to test the germination index of seeds under different concentrations of polyethylene glycol (PEG)through the paper germination test.The results showed that the seed germination rate,germina-tion force,germination index,vigor index,seeding length,and seedling moisture content were decreased,the mildew rate was increased first and then decreased when the PEG concentration increased,and the root length of seedlings had a peak value at -0.6 MPa.The seeds inactivated and could not germinate when the concentra-tion of PEG was -1.2 MPa.Compared to the control,soaking in Epichlo?fermentation broth significantly(P<0.05)promoted seed germination,seedling growth,and root growth,and effectively reduced the mildew rate (P<0.05).Compared to seed soaking with Achnatherum inebrians Epichlo?fermentation broth,the seed germination index,vigor index,seedling water content,seedling length,and root length of perennial ryegrass were greater than those in the Elymus dahuricus and Festuca sinensis experiments.In short,the fermentation broth of Epichlo?had a certain effect on the germination of perennial ryegrass seeds under drought stress,and the effect of the A.inebrians fermentation broth was the strongest of the three different kinds of fermentation broth.关键词
禾草内生真菌/浸种/萌发指标/霉变率/抗旱性Key words
Epichloëgrass edophyte/seed soaking/germination index/mildew rate/drought resistance分类
农业科技引用本文复制引用
刘静,周景乐,陈振江,魏学凯,李涛,李春杰..内生真菌发酵液浸种对干旱胁迫下黑麦草种子萌发的影响[J].草业科学,2018,35(4):839-847,9.基金项目
国家重点基础研究发展规划("973"计划)(2014CB138702) ("973"计划)
国家自然科学基金(31372366) (31372366)
长江学者和创新团队发展计划(IRT_17R50) (IRT_17R50)
中央高校基本科研业务费(lzujbky-2016-bt10、lzujbky-2017-kb10) (lzujbky-2016-bt10、lzujbky-2017-kb10)
111引智基地(B12002) (B12002)