草业学报2018，Vol.27Issue(4)：56-68,13.DOI:10.11686/cyxb2017204
黄帚橐吾提取物对保护地辣椒4种病原真菌的抑制活性及其病害防效
Inhibition activities and control efficiency of extracts from Ligularia virgaurea against 4 fungal diseases of greenhouse peppers
摘要
Abstract
Because of frequent occurrence of,serious damage from,and difficulty controlling 4 fungal diseases of greenhouse-grown peppers and the desirability of identifying safe and efficient biological rather than chemical technologies for control of these fungal diseases,the effects of Ligularia virgaurea extracts against 4 the fungal pathogens were investigated.The mycelium growth rate,spore germination count,microdilution,seed germination and pot experiment methods of assessing biocontrol were all used.The extracts from L.virgaurea had significant inhibitory activity on Botrytis cinerea,Fusarium oxysporum,Fusarium solani and Phytophthora capsici,and the inhibitory activity was positively correlated with concentration.The EC50 for mycelium inhibition was,respectively,11.93,51.25,19.93,and 13.84 g · L-1,while the corresponding EC50 values for inhibition of spore germination rate were 15.05,55.26,57.86,16.50 g · L-1,the minimum inhibitory concentration was 0.83-1.75 g · L-1,and the minimum bactericidal concentration was 2.50-11.67 g · L-1.The extracts from L.virgaurea exhibited visually obvious control efficacy on B.cinerea,F.oxysporum,F.solani and P.capsici.The preventive effect of a 100-fold diluent of the initial L.virgaurea extract was 75％ of that noted above,while the control effect was up to 70％,and was similar to the effect of commonly used chemical fungicides.However,to some extent,the extracts from L.virgaurea also inhibited pepper seed germination and establishment.The inhibition of seed germination was above 52％ when concentration was higher than 20.00 g · L-1,and the seedling height,root length,and biomass all showed significant reduction compared with the control (P＜0.05).These results show that extracts from L.virgaurea provide broad-spectrum control against frequently-occurring fungal diseases of peppers.Prospects for development of a commercial product are good.关键词
黄帚橐吾提取物/辣椒/真菌病害/防治效果Key words
extracts from Ligularia virgaurea/pepper/fungal diseases/control efficiency引用本文复制引用
李晶,李娜,丁品,杨海兴,刘锦霞,武建荣,杜文静,张建军..黄帚橐吾提取物对保护地辣椒4种病原真菌的抑制活性及其病害防效[J].草业学报,2018,27(4):56-68,13.基金项目
甘肃省科技支撑计划项目(1504GKCA074),甘肃省青年科技基金计划项目(1606RJYA292)和甘肃省科学院应用开发计划项目(2015-JK-04)资助. （1504GKCA074）
