草业学报2018，Vol.27Issue(4)：79-88,10.DOI:10.11686/cyxb2017213
红三叶遗传图谱构建及抗白粉病基因QTL定位
Construction of the AFLP linkage map and QTL analysis of powdery mildew resistance in red clover
摘要
Abstract
In this study,F1 generation red clover plants,obtained from crossing red clover varieties resistant (Gannong PR1,and Sensation and Renegade from Australia) or sensitive (Minshan) to powdery mildew,were artificially inoculated with powdery mildew and used as a quantitative trait locus (QTL) mapping population.AFLP markers were used to construct the genetic linkage map and the interval mapping method was used to locate the QTL for resistance to powdery mildew.This study will establish foundation to gene location and transgenosis of red clover.It was found that the linkage map based on 149 AFLP markers included 7 linkage groups (LG1,LG2,LG3,LG4,LG5,LG6 and LG7).The map covers a total distance of 640.5 cM.LG1 was found to be the largest (140.6 cM with the average distance between two markers being 9.4 cM),while LG4 was found to be the smallest yet with the highest marker density of any LG (55.2 cM in length with 1.8 cM on average between markers.Five QTLs (qrp-1,qrp-2,qrp-3,qrp-4 and qrp-5) related to powdery mildew resistance,and located on LG4 and LGS,were detected.The phenotypic variance explained by the 5 QTLs for powdery mildew resistance ranged from 29％ to 90％,with the largest contribution being from qrp-1.Therefore,qrp-1 was identified as the main gene controlling powdery mildew resistance in red clover.关键词
红三叶/AFLP分子标记/遗传连锁图谱/抗白粉病/QTL分析Key words
red clover (Trifolium pratense)/AFLP marker/genetic linkage map/powdery mildew resistance/QTL analysis引用本文复制引用
蒲小剑,田久胜,田新会,杜文华..红三叶遗传图谱构建及抗白粉病基因QTL定位[J].草业学报,2018,27(4):79-88,10.基金项目
国家自然科学基金(31760702,31360577),甘肃省草地畜牧业可持续发展创新团队项目(2017C-11)和现代农业产业技术体系建设专项资金(CARS-40-09B)资助. （31760702,31360577）
