重庆医学2018，Vol.47Issue(13)：1712-1715,4.DOI:10.3969/j.issn.1671-8348.2018.13.003
p53、p21在氟中毒雌鼠卵巢早衰中的表达变化
The expression changes of p53 and p21 in female rats of premature ovarian failure in fluorosis
摘要
Abstract
Objective To investigate the expression changes of p53 and p21 in premature ovarian failure in female rats exposed to coal burning fluorosis.Methods Ablactation 24 SD female rats were chosen to establish animal model (the model group),in fluorine 90 days were put to death.Observed the changes of the teeth of the female rats during fluorine exposure and ovarian granulosa cell premature aging change,detected fluorine contents of urine and bone,and expression of the ovarian granular cell gene p53 and p21.Results In the model group,fluorine spot tooth,the fluorine contents of urine and bone increased significantly.In the model group,there was no premature aging of ovarian granulosa cells in the low fluorine group,but with the increase of dye fluorine metering,ovarian granulosa cells became mild,moderate to severe progressive edema trends,cells morphological damaged fuzzy,and atresia follicles increased significantly,corpus luteum degraded,mature follicle significantly reduced,and the ovarian function gradually signs of premature aging presented.With the increase of dye fluoride measurement,the expression of aging gene p53 and p21 in each group gradually increased,compared with the control group,there was statistically significant difference (P＜0.05).Conclusion Premature ovarian failure caused by coal-burning fluorosis was significantly related to the expression of genes p53 and p21.关键词
氟化物中毒/卵巢功能早衰/p53/p21Key words
fluoride poisoning/ovarian failure, premature/p53/p21分类
医药卫生引用本文复制引用
刘洋,李艳菊,王宁,刘振华,杨华,刘燕青,王飞清..p53、p21在氟中毒雌鼠卵巢早衰中的表达变化[J].重庆医学,2018,47(13):1712-1715,4.基金项目
贵州省科技厅项目(黔科合LH字[2017]7127、7128号) （黔科合LH字[2017]7127、7128号）
贵阳中医学院基金项目([2017]14号). （[2017]14号）
