重庆医学2018，Vol.47Issue(13)：1723-1726,4.DOI:10.3969/j.issn.1671-8348.2018.13.006
miRNA-129-5p干扰技术对胆道闭锁小鼠胆道EMT作用机制的研究
Study on the mechanism of miRNA-129-5p in epithelial-mesenchymal transition of biliary atresia mouse
摘要
Abstract
Objective To investigate the mechanism of miRNA-129-5p in epithelial-mesenchymal transition (EMT)of biliary atresia.Methods Constructed bile duct epithelial EMT cell model (the experimental group) induced by TGF-β1,detected the expressions of EMT related markers and miRNA-129-5p.While miRNA-129-5p precursor was transfected,the expressions of EMT related markers and extracellular matrix were contrasted between the original and the renovated biliary epithelial cells.Results In the experimental group,extrahepatic bile duct showedEMT,the expression of miRNA-129-5p was decreased (P＜0.05),overexpression of miRNA-129-5p could inhibit the progression of EMT (P＜0.05).Conclusion miRNA-129-5p may relate to EMT by regulating the expression of TGF-β1.关键词
胆道闭锁/miRNA-129-5p/上皮间质转化Key words
biliary atresia/miRNA-129-5 p/epithelium-mesenchymal transition分类
医药卫生引用本文复制引用
王建尧,王斌,吴宙光,叶晓烁,陈子民,冯奇,刘冬,姚君..miRNA-129-5p干扰技术对胆道闭锁小鼠胆道EMT作用机制的研究[J].重庆医学,2018,47(13):1723-1726,4.基金项目
广东省医学科研基金(B2014360) （B2014360）
广东省深圳市科技创新委员会科技计划项目(JCYJ20160429174927286,JCYJ20150403101028164). （JCYJ20160429174927286,JCYJ20150403101028164）
