重庆医学2018，Vol.47Issue(13)：1727-1730,4.DOI:10.3969/j.issn.1671-8348.2018.13.007
MEK1/2抑制剂PD98059对ox-LDL诱导HUVEC损伤的保护机制及对LOX-1表达影响
The protective mechanisms of MEK1/2 inhibitor PD98059 on ox-LDL induced injury of HUVEC and the influence on the expression of LOX-1
摘要
Abstract
Objective To investigate the protective mechanism of MEK1/2 inhibitor PD98059 on ox-LDL induced injury of human umbilical vein endothelial cells (HUVEC),and its influence on the expression of LOX-1.Methods HUVEC damage models were established by using ox-LDL and were treated with PD98059 later,divided into the negative control group,the ox-LDL group,the positive control group and the PD98059+ox-LDL group.The effect of inhibition of MEK1/2 on ox-LDL induced HUVEC damage was measured.Results Compared with the negative control group,the levels in the ox-LDL group of LOX-1,pMEK1/2,RhoA,ROCK1,ROCK2,TNF-α and IL-6 were increased significantly,the proliferations of HUVEC and the productions of NO were decreased (P＜0.05).Compared with the ox-LDL group,the levels in the positive control group and the PD98059+ox-LDL group of pMEK1/2,RhoA,ROCK1,ROCK2,TNF-α and IL-6 were decreased,the proliferation of HUVEC and the production of NO were increased (P＜0.05).Conclusion PD98059 inhibit the MEK1/2 signaling pathway to suppress the ox-LDL induced damage of HUVEC by decreasing the expression of LOX-1.关键词
脂蛋白类,LDL/受体,LDL/PD98059/MEK1/2/RhoA/ROCKKey words
lipoproteins, LDL/receptors, LDL/PD98059/MEK1/2/RhoA/ROCK分类
医药卫生引用本文复制引用
孙珊,胡红玲,段晓宇,吴钦钦,贺映侠,卜晓芬,明小燕,严凤琴,朱虹..MEK1/2抑制剂PD98059对ox-LDL诱导HUVEC损伤的保护机制及对LOX-1表达影响[J].重庆医学,2018,47(13):1727-1730,4.基金项目
湖北省武汉市卫生计生科研重点项目(WX16B06) （WX16B06）
湖北省武汉市中心医院博士基金(YB16A04). （YB16A04）
