福建农业学报2018,Vol.33Issue(2):131-135,5.DOI:10.19303/j.issn.1008-0384.2018.02.005
白及种子非共生培养高效萌发体系的研究
Efficient Germination System in Asymbiotic Culture of Bletilla Striata (Thunb.) Reichb.F.
摘要
Abstract
The mature capsule of Bletilla striata was used as material for asymbiotic germination in this experiment.With MS as basal medium,effects of different sowing ways,seedling rates,storage ways,light conditions and additives on the seed germination of Bletilla striata were studied.The results showed that the best germination conditions for asymbiotic culture of Bletilla striata is with seedling rate 5 × 103 grains · mL-1 in suspended liquid,and cultured in dark conditions for the first 14 days,the germination rate of which reached 93%.Adding 10% mashed potato or coconut juice could increase the germination rate,and promote the growth of protocorrn.The seeds of Bletilla striata stored in plastics bags at 4℃ was beneficial for germination,the germination rate for which could reach 50% after 60 days.关键词
白芨/种子/非共生培养/萌发Key words
Bletilla striata/seed/asymbiotic culture/germination分类
农业科技引用本文复制引用
陈菁瑛,万学锋,刘保财,赵云青,黄颖桢,张武君..白及种子非共生培养高效萌发体系的研究[J].福建农业学报,2018,33(2):131-135,5.基金项目
福建省农业科学院“十三五”科技创新团队——药用植物创新团队项目(2017) (2017)
福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1018-1、2016R1017-1) (2014R1018-1、2016R1017-1)