福建农业学报2018，Vol.33Issue(2)：164-170,7.DOI:10.19303/j.issn.1008-0384.2018.02.011
山药组培快繁技术条件的优化
Optimization of Technical Conditions for Tissue Culture and Rapid Propagation of Chinese Yam
摘要
Abstract
With 'Ming Huai yam 2' as the experimental material,we optimized the technological conditions of the rapid propagation of yam.The results showed that in vitro culture,the best explant was segment with bud in middle part of new shoot.The best disinfection method was with 70％ alcohol immersion 10 s,and 0.1％ HgCl2 for 10 min,the pollution rate of which was 15.0％,and the mortality rate was 6.7％.The primary culture medium was;MS+ 6-BA 1.0 mg+ NAA 0.05 mg · L-1 + PVP 100 mg · L-1 + VC 500 mg · L-1 + AC 0.5 g · L-1 + Sug 30 g · L 1.After two weeks,it was transferred to the solid medium with the same formula.The browning rate was 36.7％ and the induction rate was 81.7％.The subculture multiplication medium was:MS+6-BA 2.0 mg · L 1 + KT 1.0 mg· L 1+NAA 0.05 mg · L-1 +PP333 0.05 mg· L-1 +Agar 4.0 g· L-1 +Sug 30 g · L-1.The proliferation coefficient of 30 d was 2.97.The rooting medium was:1/2MS+ NAA 0.3 mg · L-1 + IBA 0.2 mg· L-1+AC1.0g· L-1+Agar4.Sg· L-1+Sug20g· L-1.The rooting rate was 91.3％.After two weeks of acclimation in the greenhouse,the plants were soaked with carbendazim and cooled to dry,then transplanted to peat soil tray.The transplanting survival rate was more than 70％.关键词
山药/组织培养/快繁体系/优化Key words
chinese yam/tissue culture/rapid propagation system/optimization分类
农业科技引用本文复制引用
陈芝华,华树妹,李丽红,罗晓锋,贺佩珍..山药组培快繁技术条件的优化[J].福建农业学报,2018,33(2):164-170,7.基金项目
福建省农业引导性(重点)项目(2017N0051) （重点）
福建省星火科技计划项目(2016S0035) （2016S0035）
