福建农业学报2018，Vol.33Issue(2)：195-198,4.DOI:10.19303/j.issn.1008-0384.2018.02.016
琯溪蜜柚亚洲柑橘叶点霉与首都叶点霉ITS-RFLP快速鉴定
A Rapid Identification Method for Phyllosticta citriasiana and Phyllosticta capitalensis in Citrus grandis by ITS-RFLP
摘要
Abstract
Phyllosticta citriasiana is a new pathogen of Citrus grandis which causes black spot on the fruits,while Phyllosticta capitalensis is another fungus which is saprophytic and seprated from Citrus grandis.For distinguishing from each other,a rapid identification method named ITS-RFLP was established in this research.First of all,the target pathogens were seperated from the desease samples which were collected from the Citrus grandis Osbeck orchard at Pinghe city in Fujian.Then the mycelium total DNA were extracted for amplifying the rDNA-ITS gene fragments by using ITS1 and ITS4 primers.And then the amplification products were digested by the restriction enzyme of Acc Ⅰ.The results showed that the rDNA-ITS of P.citriasiana was digested into two fragments,and that of P.capitalensis into three fragments.Thus,the two kinds of fungi can be distinguished from each other by the fragment number and size.This technology is quite rapid and simple.关键词
琯溪蜜柚/黑斑病/限制性内切酶片段长度多态性分析技术Key words
Citrus grandis (L.) Osbeck/Citrus black spot/ITS-RFLP分类
农业科技引用本文复制引用
罗金水,郑域茹,卢松茂,林智明,林艺华..琯溪蜜柚亚洲柑橘叶点霉与首都叶点霉ITS-RFLP快速鉴定[J].福建农业学报,2018,33(2):195-198,4.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2012GY01) （2012GY01）
福建省自然科学基金(2010J01113) （2010J01113）
