分析测试学报2018，Vol.37Issue(4)：419-426,8.DOI:10.3969/j.issn.1004-4957.2018.04.006
超高效液相色谱-三重四极杆质谱法测定保健食品中30种非法添加壮阳类化合物
Determination of 30 Anti-impotence Compounds Illegally Adulterated in Health Products by Ultrahigh Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry
摘要
Abstract
An analytical method was developed for the determination of 30 anti-impotence compounds illegally adulterated in health products by ultra high performance liquid chromatography-triple quadrupole mass spectrometry(UHPLC-MS/MS) with a Waters CORTECS T3(100 mm ×2.1 mm,2.7 μm) column using 0.1％ (by volume) fomic acid aqueous solution-acetonitrile containing 0.1％ (by volume) formic acid as mobile phases by gradient elution.The compounds were detected with electrospray ion source in positive mode under multiple reaction monitoring (MRM) mode.The calibration curves for 30 targeted compounds were linear in the mass concentration range of 10-200 μg/L with correlation coefficients more than 0.99.The mean recoveries at spiked levels of 0.1,0.2,1.0 mg/ kg were in the range of 62.8％-135.2％,with RSDs(n =6) not more than 14％.The limits of detection(LODs) and the limits of quantitation(LOQs) were 0.05 mg/kg and 0.1 mg/kg,respectively.The established method is accurate and simple,and could be used as a reference for the national standard.关键词
超高效液相色谱-三重四极杆质谱/非法添加/壮阳类化合物/保健食品Key words
ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS)/illegal adulteration/anti-impotence compounds/health products分类
化学化工引用本文复制引用
孙健,胡青,张甦,冯睿,于泓,张静娴,诸艳蓉,毛秀红,季申..超高效液相色谱-三重四极杆质谱法测定保健食品中30种非法添加壮阳类化合物[J].分析测试学报,2018,37(4):419-426,8.基金项目
2017年国家食品药品监督管理总局食品补充检验方法项目(食品中那非类物质的筛查) （食品中那非类物质的筛查）
上海市科委技术平台专项(14DZ2294000) （14DZ2294000）
2017年上海市食品药品监督管理局课题(ZX-201710170017) （ZX-201710170017）
