分析测试学报2018，Vol.37Issue(4)：446-451,6.DOI:10.3969/j.issn.1004-4957.2018.04.010
超高效液相色谱-串联质谱法快速测定火锅底料中的5种罂粟壳生物碱残留
Rapid Determination of Five Alkaloids of Poppy Husk in Hot Pot Soup by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
李兴根 1乔勇升 2陈伟 2冯寅洁 2朱渊 2胡慧2
作者信息
- 1. 泰州市产品质量监督检验院,江苏泰州225300
- 2. 泰州市食品药品检验所,江苏泰州225300
- 折叠
摘要
Abstract
A method of ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 5 alkaloids residues of opium poppy in hot pot soup,including morphine,codeine,thebaine,papaverine and narcotine.The samples were extracted with acetonitrile containing 0.2％ fomic acid,and then analyzed by UPLC-MS/MS.The separation was carried out using a Waters ACQUITY UPLCTM BHE C18 (1.7 μm,2.1 mm × 50 mm) column by gradient elution with ammonia solution(pH 8.0) and acetonitrile as mobile phases at a flow rate of 0.3 mL/min.The five alkaloids were determined in electrospray positive ionization (ESI +) mode under multiple reaction monitoring (MRM),and the analytes were quantified by the matrix-matched standard solution.Under the optimized detection conditions,the calibration curves are linear in the ranges of 0.25-100 μg/L for morphine and codeine,0.05-100 μg/L for thebaine,and 0.05-10 μg/L for papaverine and narcotinum,respectively,with correlation coefficients all more than 0.999.The limits of detection (LODs) and quantitation (LOQs) were in the ranges of 0.9-4.5 μg/kg and 3.0-15.0 μg/kg,respectively.The average recoveries at the spiked levels of 1,5,10 μg/kg(5,25,50 μg/kg for morphine and codeine) ranged from 70.7％ to 109.6％,with relative standard deviations(RSDs) not more than 11％.This method is simple and sensitive,and is suitable for the determination and confirmation of five alkaloids residues of opium poppy illegally added in hot pot soup.关键词
固相萃取/超高效液相色谱-串联质谱(UPLC-MS/MS)/罂粟壳/罂粟碱/吗啡/火锅底料Key words
solid phase extraction(SPE)/ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/poppy husk/papaverine/morphine/hot pot soup分类
化学化工引用本文复制引用
李兴根,乔勇升,陈伟,冯寅洁,朱渊,胡慧..超高效液相色谱-串联质谱法快速测定火锅底料中的5种罂粟壳生物碱残留[J].分析测试学报,2018,37(4):446-451,6.
