分析测试学报2018，Vol.37Issue(4)：464-470,7.DOI:10.3969/j.issn.1004-4957.2018.04.013
超高效液相色谱-串联质谱法同时测定化妆品中13种性激素含量
Simultaneous Determination of Thirteen Sex Hormones in Cosmetics by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An ultra-performance liquid chromatography-tandem mass spectrometric (UPLC-MS/ MS) method was established for the determination of thirteen sex hormones in cosmetics.Toner,oil,cream and mask cosmetic samples were extracted with 50％ acetonitrile by vortex,and separated on a C1s column.The analysis was performed under dynamics multiple reaction monitoring (DMRM) mode.The results showed that the limits of detection(LODs) for 13 sex hormones in various kinds of cosmetics were in the range of 0.001-0.026 μg/g.Good linear relationship existed between peak area and mass concentration of 0.89-64.80 μg/L with correlation coefficients higher than 0.995.The average recoveries were in the range of 80.8％-116.8％,with relative standard deviations(RS Ds,n =6) of 0.7％-14.4％.With the advantages of high efficiency,sensitivity and accuracy,this method fills the gaps of determining chlormadinone acetate and other sex hormones,and is suitable for the general detection of neutral sex hormones in cosmetics.关键词
超高效液相色谱-串联质谱法(UPLC-MS-MS)/化妆品/性激素Key words
UPLC-MS/MS/cosmetics/sex hormones分类
化学化工引用本文复制引用
茹歌,王柯,陈丹丹,郑荣..超高效液相色谱-串联质谱法同时测定化妆品中13种性激素含量[J].分析测试学报,2018,37(4):464-470,7.
