高等学校化学学报2018，Vol.39Issue(5)：883-888,6.DOI:10.7503/cjcu20170548
转基因抗虫蛋白Cry1Ac与表面活性剂作用的芘探针光谱分析
Extrinsic Fluorescent Probe Analysis for Interactions Between Transgenic Insect-resistant Protein Cry1Ac and Surfactants by Pyrene
摘要
Abstract
The characteristics of interactions between the transgenic insect-resistant protein(Cry1Ac)and four different surfactants were investigated by fluorescence of pyrene,an extrinsic fluorescent probe. While the bo-vine serum albumin(BSA)was used as a reference protein. The results show that the fluorescence behavior of pyrene in RL and Tween 80 systems has a similar change pattern, and the two surfactants can be associated with proteins stably. The hydrophily and molecular polarity of Cry1Ac protein are of high values,which lead to higher I1/I3values of pyrene when the surfactant concentration is low. After the surfactant micelles formed, neither of the proteins will change the binding sites of pyrene with the nonionic or anionic surfactants. However,CTAB has a quenching effect on fluorescence of pyrene, and it cannot form stable micelles in the presence of Cry1Ac protein.关键词
芘/荧光/表面活性剂/Cry1Ac/牛血清蛋白/微环境Key words
Pyrene/Fluorescence/Surfactant/Cry1Ac/Bovine serum albumin(BSA)/Microenvironment分类
化学化工引用本文复制引用
梁运姗,黄致君,柳亦松,钱子妍,颜碧玥,王智..转基因抗虫蛋白Cry1Ac与表面活性剂作用的芘探针光谱分析[J].高等学校化学学报,2018,39(5):883-888,6.基金项目
国家自然科学基金(批准号:41401358,31472017)、中国博士后科学基金(批准号:2013M542112)和湖南省自然科学基金(批准号:2017JJ3100)资助. Supported by the National Natural Science Foundation of China(Nos.41401358, 31472017), the China Postdoctoral Science Foundation (No.2013M542112)and the Natural Science Foundation of Hunan Province, China(No.2017JJ3100). （批准号:41401358,31472017）
