高等学校化学学报2018，Vol.39Issue(5)：934-940,7.DOI:10.7503/cjcu20170464
铁皮石斛多糖的初级结构分析
Primary Structural Analysis of Dendrobium officinale Polysaccharide
摘要
Abstract
A homogeneous polysaccharide(SDOP)was obtained from the fresh Dendrobium officinale. The relative molecular mass and composition of monosaccharide were then determined by gel permeation chromatog-raphy-laser scatter(GPC-LS)and high performance liquid chromatography with pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone(PMP)as derivatization agent. The detailed chemical structure of SDOP was elucidated by means of infrared spectroscopy,methylation analysis and nuclear magnetic resonance spec-troscopy(NMR).The result shows that SDOP with an Mwof 1.66×106consists dominantly of mannose and glu-cose in a molar ratio of 4.9:1.0. The results of NMR and methylation analysis suggest that SDOP is a 1,4-linked O-acetylated glucomannan, with a small amount of branch and end signal, while acetyl groups are mainly attached to the O-2 or O-3 positions on saccharide ring.关键词
铁皮石斛/多糖/结构分析/核磁共振Key words
Dendrobium officinale/Polysaccharide/Structure analysis/Nuclear magnetic resonance分类
化学化工引用本文复制引用
高云霄,胡小龙,王月荣,姜志宏,章弘扬,张敏,胡坪..铁皮石斛多糖的初级结构分析[J].高等学校化学学报,2018,39(5):934-940,7.基金项目
澳门科技大学中药质量研究国家重点实验室开放课题(批准号:MUST-SKL-2016-06)资助. Supported by the State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, China (No.MUST-SKL-2016-06). （批准号:MUST-SKL-2016-06）
