广西医科大学学报2018，Vol.35Issue(4)：426-430,5.DOI:10.16190/j.cnki.45-1211/r.2018.04.002
氯化两面针碱通过细胞色素c介导的线粒体凋亡途径诱导人结肠癌HT29细胞凋亡
Nitidine chloride induced colorectal cancer HT29 cells apoptosis through the cytochrome c-mediated mitochondrial apoptotic pathways
摘要
Abstract
Objective:To preliminarily explore the effect of nitidine chloride (NC) on apoptosis of HT29 colorectal cancer cell line and the related mechanism.Methods:HT29 cells were divided into vehicle control group and NC groups (15,30 and 60 μmol/L).CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis,respectively.Western blotting was employed to determine the protein levels of cytochrome-c (Cyt-c) and caspase-3.Results:Compared with the vehicle control group,the cell viability rates of the NC groups decreased obviously and the apoptosis rates [(35.30±14.30)％,(41.48±17.89)％ and (46.51 ± 11.35) ％] increased prominently,and differences were statistically significant (P ＜0.05).Western blot showed that the protein levels of Cyt-c and caspase-3 in the NC groups were notably upregulated compared with the vehicle control group (P＜0.05).Conclusion:NC could significantly inhibit the proliferation of HT29 cells and induce apoptosis,and the mechanism might be associated with the Cyt-c mediated mitochondrial apoptotic pathways.关键词
氯化两面针碱/人结肠癌/线粒体凋亡途径/Cyt-c/caspase-3Key words
nitidine chloride/human colorectal cancer cells/mitochondrial apoptotic pathways/Cyt-c/caspase-3分类
医药卫生引用本文复制引用
黄燚燚,秦悦,谢雪平,傅宣皓,黄丹,刘华钢,赖泽锋..氯化两面针碱通过细胞色素c介导的线粒体凋亡途径诱导人结肠癌HT29细胞凋亡[J].广西医科大学学报,2018,35(4):426-430,5.基金项目
国家自然科学基金资助项目(No.81260657) （No.81260657）
