广西医科大学学报2018，Vol.35Issue(4)：430-435,6.DOI:10.16190/j.cnki.45-1211/r.2018.04.003
MicroRNA-451和microRNA-503对羟基脲诱导的K562细胞γ珠蛋白基因表达的调控作用
The regulation effect of microRNA-451 and microRNA-503 on γ globin gene expression in K562 cells induced by hydroxyurea
摘要
Abstract
Objective:To study the effects of hydroxyurea on γglobin gene expression in K562 cells and the regulation of microRNA that may be involved.Methods:K562 cells were treated with different concentrations of hydroxyurea (0 μmol/L,100 μmol/L,200 μmol/L,300 μmol/L,and 400 μmol/L) for 24 h,48 h,72 h and 96 h.Real-time fluorescent quantitative PCR (qPCR) was used to detect the expression of γ globin and relevant microRNA.Results:The expression of γ globin gene,miR-451a and miR-503-3p in K562 cells were significant increased in the hydroxyurea groups,compared with the control group (P＜0.05 or P ＜0.01).Conclusion:Hydroxyurea could up-regulate γ globin gene expression in K562 cells,probably though the modulation of miR-451a and miR-503-3p expressions.关键词
羟基脲/K562细胞/γ珠蛋白基因/microRNAKey words
hydroxyurea/K562 cell/γ globin gene/microRNA分类
医药卫生引用本文复制引用
盘玲媛,赖永榕,刘容容,方栋..MicroRNA-451和microRNA-503对羟基脲诱导的K562细胞γ珠蛋白基因表达的调控作用[J].广西医科大学学报,2018,35(4):430-435,6.基金项目
国家自然科学基金资助项目(No.81460025) （No.81460025）
