广西医科大学学报2018，Vol.35Issue(4)：441-445,5.DOI:10.16190/j.cnki.45-1211/r.2018.04.005
多西环素对大鼠烟曲霉菌性角膜炎中炎症细胞及MCP-1的抑制作用
Inhibitory effect of doxycycline on the inflammatory cells and the expression of MCP-1 in rats with Aspergillus fumigatus keratitis
摘要
Abstract
Objective:To investigate the effect of doxycycline on the inflammatory cells and the expression of monocyte chemoattractant protein-1 (MCP-1) in rats with Aspergillus fumigatus keratitis (AFK).Methods:Sixty-four SD rats were randomly divided into a control group and a doxycycline group,with 32 rats in each group.All rats were established the AFK model.Rats in the doxycycline group were treated with 0.3 g/L doxycycline eye-drops,and those in the control group received menstruum eye-drops.Corneal inflammatory index was recorded on the 3rd,5th,7th,and 10th day.The mRNA and protein levels of MCP-1 were detected by fluorescence quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA),respectively.Results:Compared with the control group,the inflammatory index was decreased in the doxycycline group on the 3rd,5th,and 7th day,and the inflammatory cell count was reduced at any time points (P＜0.05).The expression of MCP-1 mRNA and protein was down-regulated on the 3rd,5th and 7th day after doxycycline treatment (P＜0.05).Conclusion:Doxycycline could decrease the infiltration of inflammatory cells in rats with AFK,the mechanism might be related to the inhibition of MCP-1 expression.关键词
烟曲霉菌性角膜炎/多西环素/炎症/单核细胞趋化蛋白-1Key words
Aspergillus fumigatus keratitis/doxycycline/inflammation/monocyte chemoattractant protein-1分类
医药卫生引用本文复制引用
刘婷婷,邹文进,程璞..多西环素对大鼠烟曲霉菌性角膜炎中炎症细胞及MCP-1的抑制作用[J].广西医科大学学报,2018,35(4):441-445,5.基金项目
国家自然科学基金资助项目(No.81160119) （No.81160119）
广西自然科学基金资助项目(No.2016GXNSFAA380280 （）
No.2017GXNSFAA198347) （）
