广西医科大学学报2018，Vol.35Issue(4)：446-449,4.DOI:10.16190/j.cnki.45-1211/r.2018.04.006
淫羊藿多糖对气管切开插管留置大鼠肺部免疫功能的影响
The effects of Epimedium polysaccharides on pulmonary immune function in rats with tracheostomy
摘要
Abstract
Objective:To evaluate the effects of Epimedium polysaccharides (EPS) on pulmonary immune function in rats with tracheostomy.Methods:54 SD rats were randomly divided into three groups (n =18per group):a normal control group (group A),a model group (group B),and EPS group (group C).The rats in group B and group C were subjected to tracheotomy.After 6 h,rats in group C were treated with EPS via gavage,and those in groups A and B were treated with equal volume of saline.Secretory immunoglobulin A (sIgA) and pulmonary surfactant-related protein A (SP-A) levels in bronchoalveolar lavage fluid (BALF) and serum interleukin-2 (IL-2) level were detected by enzyme-linked immunosorbent assay (ELISA).The mRNA expression of β-defensin-2 (rBD2) mRNA in lung tissue was determined by real-time fluorescent quantitative PCR (qPCR).Results:(1) rBD-2 mRNA expression in group B was significantly higher than that in group A at 24 h after tracheostomy (P＜0.05).Compared with group B,the expression of rBD-2 mRNA in group C was decreased at 24 h,while increased significantly at 72 h and 168 h (P＜0.05).(2) The sIgA content in group B was significantly higher than that in group A after 24 h and 72 h of tracheotomy (P＜0.05),and then gradually decreased to a normal level (P＞0.05).Compared with group B,the content of sIgA in group C was notably elevated at 24 h and 168 h post-tracheotomy (P＜0.05).(3) The content of SP-A in group B was higher than that in group A at each time point,but was gradually decreased as intubation time prolonged (P＜0.05).The content of SP-A in group C was reduced at 24 h of modeling but increased with time prolonged,and was lower than that in group B at 168 h (P＜0.05).(4) The level of IL-2 in group B was higher than that in group A at 24 h post-tracheotomy (P＜0.05).Compared with group B,the serum IL-2 level in group C was markedly increased at 72 h and 168 h (P＜0.05),but there was no significant difference in IL-2 level between groups B and C at 24 h (P＞0.05).Conclusion:EPS could improve the pulmonary immune function of tracheostomy rats by regulating the rBD2 expression,elevate the contents of sIgA and SP-A in BALF and IL-2 level in serum.关键词
淫羊藿多糖/气管切开插管留置大鼠/β-防御素-2/sIgA/SP-A/IL-2Key words
Epimedium polysaccharide/tracheostomy/β-defensin-2/sIgA/SP-A/interleukin-2分类
医药卫生引用本文复制引用
彭玲玲,陈铭坚,王学敏,潘宇政..淫羊藿多糖对气管切开插管留置大鼠肺部免疫功能的影响[J].广西医科大学学报,2018,35(4):446-449,4.基金项目
国家自然科学基金资助项目(No.81460687) （No.81460687）
广西壮族自治区卫生厅中医药科技专项基金资助项目(No.GZPT13-36) （No.GZPT13-36）
