广西医科大学学报2018，Vol.35Issue(4)：454-458,5.DOI:10.16190/j.cnki.45-1211/r.2018.04.008
调控Rac1/AKT/NF-κB信号通路对呼吸机相关性肺损伤大鼠的影响
Effect of the regulation of Rac1/AKT/NF-κB signaling pathway on ventilator-induced lung injury in rats
摘要
Abstract
Objective:To explore whether Ras related C3 botulinum toxin substrate 1/protein kinase B/nuclear factor-κB (Rac1/AKT/NF-κB) pathway is involved in the process of ventilator induced lung injury (VILI) in rats.Methods:Thirty Sprague-Dawley (SD) rats were randomly divided into 3 groups (n=10 per group):spontaneous respiratory group,high tidal volume (VT) group and NSC23766 group.Rats in the NSC23766 group were given 200 μL NSC23766 through tracheal catheter for 1 h,then received mechanical ventilation with 40 mL/kg VT to establish a VILI model.The lung wet-to-dry weight ratio (W/D) was calculated.The pathological changes of lung tissue was observed.The total protein content in bronchoalveolar lavage fluid (BALF) was measured using BCA method.The concentrations of interleukin (IL)-1β and IL-6 in serum and Rac1 in BALF were determined by enzyme linked immunosorbent assay (ELISA).The mRNA expressions of NF-κB and Rac1 were detected by fluorescent quantitative PCR (qPCR).The protein expressions of AKT,phosphorylated (p)-AKT,and NF-κB were determined by western blotting.Results:No obvious pathological changes of lung tissues were found in spontaneous respiratory group,while pulmonaryalveoli fusion,alveoli septum thickening,and inflammatory cells infiltration were observed in high VT group.In NSC23766 group,the lung tissue exhibited slight edema and infiltration of inflammatory cells.Compared with the spontaneous respiratory group,the W/D ratio,the total protein and Rac1 in BALF,and inflammatory cytokines (IL-1β and IL-6) levels in serum and BALF were significantly increased in high VT group and NSC23766 group (P＜0.05),meanwhile,the expressions of NF-κB and Rac1 mRNA as well as the protein expressions of AKT,p-AKT and NF-κB were up-regulated (P＜0.05),but the changes were more significant in high VT group (P＜0.05).Conclusion:Rac1/AKT/NF-κB pathway was involved in the development of VILI.Rac1 inhibitor NSC23766 attenuated the release of inflammatory factors,thereby affecting the progress of VILI to some extent.关键词
呼吸机相关性肺损伤/Rac1/NSC23766/NF-κB/炎性因子Key words
ventilator induced lung injury/Rac1/NSC23766/NF-κB/inflammatory factors分类
医药卫生引用本文复制引用
宋世雄,潘灵辉..调控Rac1/AKT/NF-κB信号通路对呼吸机相关性肺损伤大鼠的影响[J].广西医科大学学报,2018,35(4):454-458,5.基金项目
基金项目 （）
广西自然科学基金重点项目资助(No.2014GXNSFDA118026) （No.2014GXNSFDA118026）
广西医疗卫生适宜技术研究与开发项目资助(No.S201418-02) （No.S201418-02）
