广西医学2018，Vol.40Issue(7)：803-807,5.DOI:10.11675/j.issn.0253-4304.2018.07.21
淫羊藿对气管切开插管留置大鼠免疫功能的影响
Effects of Epimedium brevicornu Maxim. on immune function of rats with trachea cannula retention after tracheostomy
摘要
Abstract
Objective To explore the effect of Epimedium brevicornu Maxim. on immune function in rats with trachea cannula retention after tracheostomy.Methods Fifty-four SD rats were randomly divided into normal control group(group A),model group without medication(group B),and model group with medication(group C),with 18 rats in each group.Rat model with tracheostomy and trachea cannula retention was established in group B and group C.After 6 h of modeling,intragastric administration with water extract of Epimedium brevicornu Maxim.was performed in group C,and intragastric administration with same volume of normal saline was performed in the other two groups.At the 24th,72th and 168th hour after intragastric administration,β-defensin-2(BD-2) mRNA expression level in the lung tissues,interleukin 2(IL-2) level and T cell subsets counts in the peripheral blood were detected in 6 rats of each group.Results (1)In group B,the relative expression levels of BD-2 mRNA at the 72th and 168th hour were lower than the level at the 24th hour(P<0.05), and the level in group B increased at the 24th hour but decreased at the 168th hour compared to the level in group A(P<0.05).At the 24th,72th and 168th hour,the relative expression levels of BD-2 mRNA were higher in group C compared to the levels in group B(P<0.05). (2)At the 72th and 168th hour,the levels of IL-2 and CD4+were higher in group C compared to the levels in group B(all P<0.05).At the 24th hour,the CD3+level in group B was lower than that in group A,but the level in group C was higher than that in group B(all P<0.05). There were no significant differences in the CD8+level at the 24th or 72th hour among the three groups(P>0.05),but the level at the 168th hour was lower in group C compared to the level in group A(P <0.05).Conclusion Epimedium brevicornu Maxim. can improve the immune function in rats with trachea cannula retention after tracheostomy by up-regulating the expression of BD-2 mRNA in pulmonary tissues and the expressions of CD4+T cell and IL-2 in blood as well as down-regulating the expression of CD4+T cell.关键词
气管切开/淫羊藿/β-防御素-2/白细胞介素-2/T细胞亚群/大鼠Key words
Tracheostomy/Epimedium brevicornu Maxim./Beta-defensin-2/Interleukin 2/T cell subset/Rat分类
医药卫生引用本文复制引用
朱翠香,彭玲玲,潘宇政,潘茂华,王学敏..淫羊藿对气管切开插管留置大鼠免疫功能的影响[J].广西医学,2018,40(7):803-807,5.基金项目
国家自然科学基金(81460687)；广西壮族自治区卫生厅中医药科技专项(GZPT13-36) （81460687）
