广西医学2018，Vol.40Issue(7)：812-817,6.DOI:10.11675/j.issn.0253-4304.2018.07.23
益髓灸对D-半乳糖衰老小鼠模型海马组织Klotho蛋白表达及胰岛素通路的影响
Effect of Yisui moxibustion on Klotho protein expression and insulin pathway of hippocampal tissues in aging mice model induced by D-galactose
摘要
Abstract
Objective To explore the effect of Yisui moxibustion on Klotho,insulin receptor substrate 1(IRS-1),protein kinase B(Akt),forkhead box O3(FOXO3a) mRNA and protein expressions of hippocampal tissues in aging mice model induced by D-galactose. Methods Sixty male Kunming mice are randomly divided into normal saline group,model group,Zusanli group,Baihui group and nonacupoint group,with 12 mice in each group.The mice in the model group and each intervention group were given subcutaneous injection of D-galactose for establishing aging mice model,and in the normal saline group received normal sodium injection with the same dosage.On the 13th day of modeling,the three intervention groups underwent moxibustion at corresponding acupoints,while the normal saline group and model group were given scratch with the same degree.Behavior test using Morris water maze was conducted in the five groups before and after modeling. After the treatment finished,the expression levels of Klotho,IRS-1,Akt and FOXO3a mRNAs and proteins were detected in hippocampal tissues of the mice.Results (1)After modeling,the latent periods of the model group and the nonacupoint group were longer than that of the normal saline group,the Zusanli group or the Baihui group(P<0.05),and no statistically significant difference was found in the latent period between the nonacupoint group and the Baihui group,or between the nonacupoint group and the Zusanli group(P>0.05).(2)In the model group and nonacupoint group,the expression levels of Klotho and IRS-1 mRNAs and proteins decreased,and the expression levels of Akt1 and FOXO3a mRNAs increased compared to the levels in the normal saline group(P<0.05).Compared to the model group,the Zusanli group and Baihui group had higher expression levels of Klotho and IRS-1 mRNAs but lower expression levels of Akt1 and FOXO3a mRNAs (P<0.05).(3)In the model group,the levels of Klotho,phospho-IRS-1(p-IRS-1),Akt and FOXO3a proteins decreased(P<0.05),and the levels of IRS-1,phospho-Akt(p-Akt) and phospho-FOXO3a(p-FOXO3a) proteins increased compared to the levels in the normal saline group(P<0.05).Compared to the model group,the Zusanli group and Baihui group obtained higher levels of Klotho,p-IRS-1,Akt and FOXO3a proteins but lower levels of IRS-1,p-Akt and p-FOXO3a proteins(P<0.05),and the nonacupoint group had a lower expression level of p-Akt protein(P<0.05).Conclusion Anti-aging effect of Yisui moxibusition on the brain might relate to:Yisui moxibusition can probably up-regulate the Klotho and IRS-1 mRNA levels to promote the expression of Klotho protein in hippocampus of aging mice model induced by D-galactose,stimulate the activation of IRS-1 serine inhibition site,down-regulate its downstream Akt activity and thus down-regulate of phosphorylation expression of its substrate FOXO3a.关键词
阿尔兹海默病/益髓灸/Klotho/胰岛素通路/D-半乳糖/脑衰老/小鼠Key words
Alzheimer′s disease/Yisui moxibostion/Klotho/Insulin pathway/D-galactose/Brain aging/Mouse分类
医药卫生引用本文复制引用
赵利华,陈望龙,吴萍萍,陈树燕,罗志洪,方玉丽..益髓灸对D-半乳糖衰老小鼠模型海马组织Klotho蛋白表达及胰岛素通路的影响[J].广西医学,2018,40(7):812-817,6.基金项目
国家自然科学基金(81360561) （81360561）
