广西植物2018，Vol.38Issue(4)：482-491,10.DOI:10.11931/guihaia.gxzw201706026
'章姬'草莓茎段愈伤组织诱导及高频植株再生体系的建立
Efficient callus induction and high?frequency regeneration system establishment of strawberry 'AKihime'
摘要
Abstract
In order to cultivate an improved variety with higher potential yield and establish an efficient in vitro regeneration system of strawberry'Akihim'(Fragaria ×ananassa),callus induction and plant regeneration protocol was designed for solve browning problem.A formal L9(34)orthogonal experiment was designed to investigate the browning research in pri?mary culture using explants of creeping stems excised from aseptic seedlings.Based on the improved medium above,single?factor experiments were conducted to select effective plant growth regulators and appropriate concentrations on blank MS medium.A L9(34)orthogonal experiment was designed to study effects of types and concentrations of plant growth regulators on callus induction,adventitious shoot formation,and plant regeneration.The results indicated that MS medium was inferior to B5and 1/2MS in inhibiting browning condition.The browning rate was dramatically reduced while the callus still survived on the medium with the addition of 20 g·L?1Na2S2O3. The callus induction and shoot formation of the explants were ob?served on MS + 0.1 mg·L?16?BA+0.05 mg·L?12,4?D+0.1 mg·L?1NAA with effective inhibiting browning.The opti?mal medium protocol for multiple shoots proliferation was MS+ 0.1 mg·L?16?BA+ 0.1 mg·L?1NAA where the prolifera?tion coefficient was 12.86 after 30 d.Healthily regenerated plants were yielded on culture medium 1/2MS+ 1.0 g·L?1AC after 35 d,with a rooting rate of 92.50%.More than 95%of plantlets survived after transplanting into field.The rapid propa?gation system is helpful to provide homogeneous progeny and high quality seedlings for cultivation of strawberry'Akihime' as well as a technical reference for other strawberry species in vitro regeneration.关键词
'章姬'草莓/匍匐茎/正交实验/褐化/繁殖系数Key words
strawberry'Akihime'/creeping stem/orthogonal test/browning/propagation coefficient分类
农业科技引用本文复制引用
张爱丽,王元忠,黄衡宇,李继祥,马利娟..'章姬'草莓茎段愈伤组织诱导及高频植株再生体系的建立[J].广西植物,2018,38(4):482-491,10.基金项目
国家自然科学基金(31260077) （31260077）
云南省教育厅重点项目(2015119)[Supported by the National Natural Science Foundation of China(31260077) （2015119）
Educational Commission of Yunnan Province(2015119)]. （2015119）
