广州化学2018，Vol.43Issue(2)：72-75,4.DOI:10.16560/j.cnki.gzhx.20180210
血液中4种巴比妥类药物的固相萃取及液相质谱法分析
Analysis Method of Four Types of Barbiturates by SPE and Liquid Chromatography-Mass Spectra
柯尊和1
作者信息
- 1. 广东华生司法鉴定中心,广东 广州 510455
- 折叠
摘要
Abstract
A solid phase extraction (SPE) method for the determination of four barbiturates such as barbiturate, phenobarbital, isopentobarbital and sparkobarbital (Sparbital) in blood was established. The acid, neutral and alkaline extraction conditions were studied and several different solid phase extraction columns were selected. The results showed that the pH=4 acid extraction condition and HLB solid phase extraction column was better. The minimum detection limit of of the four barbiturates was barbiturate (5 ng/mL), phenobarbital (10 ng/mL), isopentobarbital (2 ng/mL), secobarbital (5 ng/mL), and the linear range was 10~250 ng/mL. The method was simple and easy to operate, and the recovery rate was high, which provided a reference for the qualitative and quantitative analysis of the blood barbiturates.关键词
巴比妥类药物/固相萃取/液质联用Key words
barbiturate/solid phase extraction/liquid chromatography-mass spectra分类
化学化工引用本文复制引用
柯尊和..血液中4种巴比妥类药物的固相萃取及液相质谱法分析[J].广州化学,2018,43(2):72-75,4.
