华北农学报2018，Vol.33Issue(2)：58-64,7.DOI:10.7668/hbnxb.2018.02.009
杏扁PaCBF1基因的克隆及其生物信息学分析
Cloning and Bioinformatic Analysis of PaCBF1 Gene in Prunus armeniaca L.
摘要
Abstract
The C-repeat binding factors (CBF)/dehydration-responsive element-binding protein (DREB) are a group of conserved transcription factors that play an important role in the response and adaptation to environmental stress in many plants. In order to investigate the role of CBF transcription factors in plant abiotic stresses of Prunus armeniaca L.,a CBF/DREB1 transcription factor gene was cloned using reverse transcription-PCR(RT-PCR) from Weixuan No.1 and designated as PaCBF1. The GenBank accession number was MF491392. Bioinformatics analy-sis was carried out and the results showed that:The open reading frame(ORF) of PaCBF1 was 723 bp,which en-coded a protein of 240 amino acids with a molecular weight of 26.928 ku and a pI of 6.35. The grand average of hydropathicity was-0.555.PaCBF1 protein contained an AP2 DNA-binding domain and two CBF signature se-quences that one was PKKRAGRRVFKETRHP and the other was DSAWR. The prediction of secondary structure of PaCBF1 protein indicated that the encoded protein mainly contained 38.75% of random coil,29.58% of α-helix, 22.92% of extended strand and 8.75% of β-turn. Tertiary structure of PaCBF1 protein contained α-helix,β-plea-ted sheet and β-turn. PaCBF1 protein was localized in the nucleus,it did not contain a signal peptide,and it was not a secreted protein. PaCBF1 protein contained 13 phosphorylation sites. These results provided a theoretical ba-sis for the further study of the function of the kernel apricot CBF transcription factor and the resistance mechanism to the stress environment.关键词
杏扁/PaCBF1/基因克隆/生物信息学Key words
Kernel apricot/PaCBF1/Gene clone/Bioinformatics分类
生物科学引用本文复制引用
王连荣,薛拥志,王宝地..杏扁PaCBF1基因的克隆及其生物信息学分析[J].华北农学报,2018,33(2):58-64,7.基金项目
河北省高等学校科学技术研究项目(NQ2015202) （NQ2015202）
河北科技厅项目(17226303D) （17226303D）
张家口科技局项目(1611047C) （1611047C）
