华北农学报2018，Vol.33Issue(2)：72-78,7.DOI:10.7668/hbnxb.2018.02.011
基于VIGS技术的小麦干旱响应基因TaEF-1α的功能研究
VIGS-based Functional Analysis of Wheat Drought Responsive Gene TaEF-1α
摘要
Abstract
The expression level of the protein product of TaEF-1α gene was up-regulated under drought stress condition in one of our previous study using iTRAQ technology. To further study the function of TaEF-1α gene in response to drought stress in wheat,we quantified the expression of TaEF-1α gene at the transcriptional level gene under normal and drought stress conditions,the expression level of TaEF-1α gene was knock down by using virus induced gene silencing (VIGS) technique to study its function. The results showed that the expression of TaEF-1α gene was up-regulated by drought stress. Under drought stress condition,the expression level of TaEF-1α gene and the relative water content of leaves decreased significantly and the relative conductivity and malondialdehyde (MDA) content increased significantly in knock-down-plants than wild type plants. The changes in phenotypes and physiological indices of wheat plants after drought stress and VIGS silencing TaEF-1α gene indicated that the drought-induced TaEF-1α gene expression in wheat plants was up-regulated, and the TaEF-1α gene knock-down-plants also exhibited leaf drooping earlier and more sensitive to drought stress than wild type plants. Taken togeth-er,TaEF-1α gene may play a crucial role in response to drought stress in wheat.关键词
小麦/干旱胁迫/TaEF-1α基因/VIGS技术Key words
Wheat/Drought stress/TaEF-1α gene/VIGS technique分类
生物科学引用本文复制引用
王新博,任永哲,岳慧芳,李乐,吕伟增,史校艳,辛泽毓,王志强,林同保..基于VIGS技术的小麦干旱响应基因TaEF-1α的功能研究[J].华北农学报,2018,33(2):72-78,7.基金项目
国家重点研发计划项目(2016YFD0300205) （2016YFD0300205）
河南省自然科学基金项目(162300410133) （162300410133）
