华北农学报2018，Vol.33Issue(2)：87-94,8.DOI:10.7668/hbnxb.2018.02.013
重瓣百合LiAGL6基因的克隆与表达分析
Cloning and Expression Analysis of LiAGL6 in Double Lily
摘要
Abstract
To explore flower development molecular mechanism of the Double lily,the AGL6 homologous gene was cloned from Double lily cv. Belonica. The open reading frame of LiAGL6 gene was 744 bp,encoded a protein of 247 amino acids. The LiAGL6 protein molecular weight was 28.3 ku,the grand average of hydropathicity was-0.748 and the theoretical pI was 8.23. The protein structure analysis showed that LiAGL6 protein had a typical MADS-box domain,a K-box region and two AGL6 motifs. Phylogenetic tree analysis showed that LiAGL6 belonged to monocotyledon group,AGL6 branch of the AP1/AGL9 subfamily,and had the highest similarity with Hyacinthus orientalis which was also belonged to monocotyledons,the similarity reached 78%. All the results indicated LiAGL6 was the homologous gene of AGL6,so named it LiAGL6. Cellular localization assay revealed LiAGL6 expressed in the nucleus of onion epidemical cells,which was the basic characteristics of the transcription factors. The qRT-PCR re-sult showed the highest expression of LiAGL6 was in flowers while no expression occurred in leaves and stems. LiA-GL6 was expressed most in the seventh whorl petals followed by the sixth and third whorl petals,however,there was almost no expression in the second whorl petals. The research showed the LiAGL6 gene might play a regulatory role on formation of Double lily flowers.关键词
重瓣百合/MADS-box/基因克隆/LiAGL6基因/表达分析Key words
Double lily/MADS-box/Gene cloning/LiAGL6 gene/Expression analysis分类
生物科学引用本文复制引用
隋娟娟,李晓昕,吴健,曹兴,吴泽,何俊娜,义鸣放..重瓣百合LiAGL6基因的克隆与表达分析[J].华北农学报,2018,33(2):87-94,8.基金项目
国家自然科学基金项目(31471904) （31471904）
安徽省教育厅自然科学研究重点项目(KJ2016A871) （KJ2016A871）
安徽省高校质量工程项目(2015jyxm221) （2015jyxm221）
