华北农学报2018，Vol.33Issue(2)：95-99,5.DOI:10.7668/hbnxb.2018.02.014
钾素对马铃薯钾转运体KUP6和KUP7基因表达量的调节
Response of Potassium Concentration on Potassium Transporter KUP6 and KUP7 Gene Expression in Potato Seedlings
摘要
Abstract
In order to study response of the potassium concentration on the expression of KUP6 and KUP7 po-tassium transporter gene,hydroponic potato seedlings was conducted.The RNA of potato was isolated and checked, the first chain of the cDNA was synthesized by the Reverse Transcriptional Kit,and gene transcription on seedlings was detected by Real-time fluorescence quantitative PCR technique and the content of potassium in potato seedlings was detected.The content of potassium in potato seedlings was detected at 10,20 and 30 days after potato seedling planting. The results showed that when potassium concentration was 1.00 mmol/L,the expression level of KUP6 and KUP7 was the highest. When potassium concentration was lower than 0.10 mmol/L,the expression was low, and the expression level of potassium decreased when potassium concentration was 10 mmol/L. It indicates that higher and lower potassium concentration is not conducive to the expression of KUP6 and KUP7 genes. Potassium content in roots, stems and leaves showed that potassium concentration reached its absorption saturation at 1.00 mmol/L for root. The higher potassium concentration in hydroponics, the higher potassium content in root, stem and leaf. The difference of potassium content in stem and leaf was decreased when lower potassium concentration in hydroponics and increased when higher concentration.关键词
马铃薯/钾转运体/qPCR/KUP6/KUP7Key words
Potato/Potassium transporter/qPCR/KUP6/KUP7分类
农业科技引用本文复制引用
张子义,赵阳,梁红胜,樊明寿..钾素对马铃薯钾转运体KUP6和KUP7基因表达量的调节[J].华北农学报,2018,33(2):95-99,5.基金项目
内蒙古自然科学基金项目(2017MS0351) （2017MS0351）
内蒙古硕士研究生科研创新资助项目(S20171012912) （S20171012912）
国家自然科学基金项目(31760356) （31760356）
