华北农学报2018，Vol.33Issue(2)：112-118,7.DOI:10.7668/hbnxb.2018.02.016
分子标记辅助选择改良水稻恢复系R389及其杂交种稻瘟病抗性
Improving Blast Resistance of Rice Restorer 389 and Its Hybrid Through Molecular Marker-assisted Selection
摘要
Abstract
To directionally improve blast resistance of the indica rice restorer R389 and its hybrid,a functional DNA marker Ins1-1 was developed according to the broad-spectrum and durable blast resistance gene Pi9,and mo-lecular marker-assisted selection(MAS) consecutive backcross breeding were implemented. Resistance responses and resistance spectra to twenty tested M.oryzae isolates were quite different between the Pi9 gene donor 75-1-127 conferring resistance to eighteen isolates and the receptor R389 which showed resistance to only eleven isolates,and the resistance frequencies were 90.0% and 55.0%,respectively in the greenhouse inoculation assay. Accordingly, 75-1-127 showed high-level blast resistance during seedling stage and panicle developing stage but R389 was highly susceptible in field nursery. Ins1-1 was a PCR-based dominant DNA marker,showed obvious and stable polymor-phism between two parents,by amplifying a 513 bp band from the genome of donor 75-1-127,the pure line of R389-Pi9 and its hybrid,but no product from that of the receptor R389 and its hybrid. Selection efficiency by Ins1-1 genotype for blast resistance phenotype reached 100%. An improved restorer line R389-Pi9 was bred from the twenty-six BC6F3family lines,which showed similar field characteristics but much better blast resistance compa-ring to R389,and its two-line hybrid Liangyou 389-Pi9(P88s/R389-Pi9)was created which showed high level seedling and panicle blast resistance in field nursery. Field cultivation assay showed that the improved pure line R389-Pi9 and its hybrid Liangyou 389-Pi9 showed no significant difference to corresponding contrasts in main agro-nomic traits such as growth period,plant height,seed setting rate and 1000-grain weight,implying similar genetic background and improved blast resistance were achieved.关键词
水稻/稻瘟病抗性改良/Pi9基因/分子标记辅助选择Key words
Rice/Blast resistance improvement/The Pi9 gene/Molecular marker-assisted selection分类
农业科技引用本文复制引用
李永聪,刘雄伦,黄俊,廖花,陈海龙,杨丰宇,赖怡帆,张旭辉,刘金灵,王国梁..分子标记辅助选择改良水稻恢复系R389及其杂交种稻瘟病抗性[J].华北农学报,2018,33(2):112-118,7.基金项目
国家转基因重大专项(2016ZX08001-002) （2016ZX08001-002）
国家重点研发计划(2016YFD0101103-2) （2016YFD0101103-2）
湖南省科技重大专项(2015NK1001-2) （2015NK1001-2）
