华北农学报2018，Vol.33Issue(2)：126-132,7.DOI:10.7668/hbnxb.2018.02.018
利用InDel指纹图谱评价鲁西南地区簇生朝天椒品种的相似度
Similarity Evaluation of Clustered Pepper Varieties in Southwest Shandong Province with InDel Fingerprint
摘要
Abstract
The agronomic traits of clustered pepper were studied in Southwest Shandong Province Area,molecu-lar fingerprinting were established,in order to support the similarity evaluation methods with molecular level and ag-ronomic traits,also to lay the foundation of varieties evaluation,varieties protection and varieties management.Eight clustered pepper varieties with different brand names were used as experimental materials,the number of single plants,fruit number per cluster,fresh weight per fruit,dry weight per fruit and color value were tested.UPGMA clus-ter analysis was used to evaluate the agronomic traits similarity among eight varieties.The genomic DNA was extrac-ted by the kit method. Eight pepper materials with different biological characters were used to select 25 polymorphic InDel primers,and establish InDel molecular fingerprinting. Using agronomic traits and DNA fingerprinting,similar-ity evaluation was carried out among eight clustered pepper varieties. Accorded to the results of agronomic traits,the eight varieties all had the characteristics of better lodging resistance,compact plant type,high yield,and consistent performance. When the genetic distance was 0.78,the eight varieties were clustered into one major category in the agronomic characters.The fingerprinting established with 25 pairs polymorphism InDel primers,160 sites were am-plified,and the amplified fragment size was 130-350 bp. In the InDel fingerprinting,there was only different in primers CIDH267 among 8 varieties,the amplified fragment size was 180-200 bp. Reference DNA fingerprinting method (NY/t1433-2007),SYJ-2,SYJ-6 and varieties SYJ-1,SYJ-3,SYJ-4,SYJ-5,SYJ-7,SYJ-8 were similar vari-eties.Based on agronomic traits and DNA fingerprinting could effectively evaluate the similarity among varieties. There were small agronomic characters differences,low level genetic diversity,higher similarity among the eight clustered pepper. So,the homogeneity and synonyms of clustered pepper was serious in this area.关键词
簇生朝天椒/相似度评价/农艺性状/InDel标记/指纹图谱Key words
Clustered pepper/Similarity evaluation/Agronomic traits/InDel Marker/Fingerprint分类
农业科技引用本文复制引用
姜童,王辉,陈宁,付翔,杨延杰,林多..利用InDel指纹图谱评价鲁西南地区簇生朝天椒品种的相似度[J].华北农学报,2018,33(2):126-132,7.基金项目
山东省农业良种工程资助项目(2016LZGC011) （2016LZGC011）
山东省自然科学基金项目(ZR2014CQ034) （ZR2014CQ034）
青岛农业大学高层次人才科研基金项目(6631115041) （6631115041）
