华北农学报2018，Vol.33Issue(2)：133-138,6.DOI:10.7668/hbnxb.2018.02.019
不同生态环境下籽粒大小相关性状QTL定位
QTL Mapping of Seed Size Traits under Different Environment in Rice
摘要
Abstract
To reveal the genetic basis of seed size traits,identification of novel QTL related to grain size under different environments,which is important to provide us with useful information for marker-assisted improvement of the higher yield and quality in rice. A recombinant inbred lines(RILs) derived from the cross between Longdao 5 (Japonica) and Zhongyouzao 8(Indica) was used to identify QTLs for seed size under multi-environments(Sheny-ang,2015;Nanchang,2016;Hainan,2016),QTLs were mapped by QTL IciMapping v4.0 software,using ICIM method for seed size traits. Analysis of variance test showed that a significant difference was detected between the parents under three different conditions. A normal distribution of phenotypic values for seed size traits were ob-served,which indicated that seed size traits were controlled by multiple genes. Thirty-five QTLs for seed size were detected on the chromosome 2,3,4,5,8,10 and 11 under three different environments,the range of individually ex-plaining phenotypic variation from 5.76% to 38.88%,eleven were stable expression across two or three environ-ments,qST8,qSL11,qSV11 and qTGW11 were detected under three environments,qSV2b,qST3,qSLW3,qSLW8,qT-GW8,qSW8 and qSV8 were detected under two environments,respectively. These QTLs clustered on the chromo-some 2,3,8 and 11,which these QTLs cluster qSS8(qSW8,qST8,qSLW8,qSV8 and qTGW8) and qSS11 (qSL11, qSV11 and qTGW11)were located on the chromosome 8 and 11 with pleiotropic genetic overlap effect and stable ex-pression,which a novel major QTL qSS8 and these research results have an obvious effects on seed size with the sig-nificant value of theoretical and breeding practice.关键词
水稻/不同环境/籽粒大小/qSS8/数量性状位点Key words
Rice/Different environment/Seed size/qSS8/QTL分类
农业科技引用本文复制引用
刘进,姚晓云,王棋,李慧,王嘉宇,黎毛毛..不同生态环境下籽粒大小相关性状QTL定位[J].华北农学报,2018,33(2):133-138,6.基金项目
江西省青年科学基金项目(20171BAB214026) （20171BAB214026）
国家重点研发计划项目(2016YFD0100101-11) （2016YFD0100101-11）
江西省农业科学院博士启动基金项目(20162CBS007) （20162CBS007）
江西省现代农业科研协同创新专项(JXXTCX201701-01) （JXXTCX201701-01）
